Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02354-0006-0000-2
Chemical Name:	Lead(2+) acetate
CASRN:	301-04-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G09316
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	89	141	46	41
			125r	119	143	38
			111	105	119	36
			118	106	150	42
Test Chemical	Lead(2+) acetate	282	109	97	129	40
			127r	121	116	30
			123r	123	120	33
		332	104	73	133	42	37
			136r	125	90	22
			115	90	107	31
		391	87	80	110	42	38
			116	93	127	37
			116	102	122	35
		460	118	87	147	42	43
			126r	92	144	38
			116	94	153	44
		541	112	66	189	57	54
			114	69	209	61
			101	65	132	44
		637	105	45	180	57	55
			104	36	168	54
			92	38	152	55
		750	74	8	154	69	63*
			83	12	140	57
			80	9	152	63
Positive Control	Ethyl Methane Sulfonate	500	67	43	1135	566	534*
			68#	44	1050	517
			69	45	1083	521

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	103	81	37	45
			83	108	115	46
			72	89	89	41
			81	100	138	57
Test Chemical	Lead(2+) acetate	42	80#	157	129	54	51
			78	138	113	48	51
		52	80	163	97	41	51
			81	151	152	62	51
		66	81	94	147	61	54
			65	62	92	47	54
		82	85	74	173	68	67
			80#	96	157.5	66	67
		102	61	61	102	56	49
			82	80	102	42	49
		128	70	67	105	50	63
			79	81	183	77	63
Positive Control	3-Methylcholanthrene	5	73	79	411	187	204*
			67	72	452	227
			67	70	395	197

Non-Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	101	135	55	52
			91	108	129	47
			81	92	148	61
			81	100	107	44
Test Chemical	Lead(2+) acetate	66	78	69	198	85	81*
			62	47	185	99
			77	56	134	58
		78	78	76	193	83	66
			84	78	118	47
			65	50	132	68
		91	73	69	145	67	70
			66	57	138	70
			65	61	144	74
		107	70	64	138	66	61
			72	76	122	57	61
		126	72	69	101	47	45
			87	87	116	44
			82	85	106	43
		149	71	64	149	70	54
			81	87	138	57
			85	93	88	35
		175	73	74	78	36	34
			86	88	90	35
			72	79	66	31
Positive Control	3-Methylcholanthrene	5	62	67	385	206	208*
Positive Control			71	65	444	209	208*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	105	213	94	92
Vehicle Control			83	95	224	90	92
Test Chemical	Lead(2+) acetate	83.9	76r	76	234	102
		105	73	77	272	124	121
			72	72	256	118	121
		131	73	68	256	117	122
			85	96	324	127	122
		164	81	94	316	131	120
			86	75	283	110	120
		205	48	53	250	172	150*
			74	73	284	129	150*
		256	86	74	340	132	122
			107	81	361	113	122
Positive Control	3-Methylcholanthrene	5	20	8	267	441	503*
Positive Control			18	6	311	565	503*

Induced S9 Trial: 2 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	99	368	141	144
Vehicle Control			81	101	358	147	144
Test Chemical	Lead(2+) acetate	83.9	97	118	322	110	107
			101	119	317	104	107
		105	109	139	340	104
			120r	144	353	98
		131	103	121	300	97
			126r	143	331	88
		164	87	26	340	131	118
			95	74	297	104	118
		205	77	39	439	189	170
			89	33	405	151	170
		256
		320
		400

Positive Control	3-Methylcholanthrene	5	30	13	480	530	500*
Positive Control			33	16	470	470	500*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	98	142	66	63
			94	118	197	70
			75	84	118	53
Test Chemical	Lead(2+) acetate	64.6	87	87	266	102	100*
			77	122	226	98	100*
		71.7	83	81	217	87	80
			87	103	188	72	80
		79.7	96	99	207	72	73
			90	109	209	78
			88	86	182	69
		88.6	89	105	218	82	85
			94	114	242	86
			76	85	195	86
		98.4	95r	106	223	79
		121.5	76r	95	127	56
Positive Control	3-Methylcholanthrene	5	42	24	464	365	377*
			41	23	497	407
			44	21	471	358

Induced S9 Trial: 4 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	110	164	67	65
			93	109	179	64
			82	86	175	71
			92	95	152	55
Test Chemical	Lead(2+) acetate	53.8	97	88	219	75	83
			99	100	271	91	83
		59.8	108	86	193	60	69
			118	109	241	68
			106	93	257	81
		66.4	90	87	163	60	66
			106	112	228	72
			112	114	225	67
		73.8	101	94	256	84	71
			113	113	231	68
			118	122	216	61
		82	109	131	249	76	69
			135r	132	333	82
			107	113	194	61
		91.1	92	104	220	80	77
			95	115	219	77
			117	120	259	74
		101.2	98	122	179	61	66
			114	88	217	64
			92	74	202	73
		112.5	99	92	209	70	79
			90	99	255	94
			109	99	236	72
		125	91	90	242	88	88
			100	108	270	90
			92	112	238	86
Positive Control	3-Methylcholanthrene	5	63	38	561	296	316*
			49	26	524	359
			56	32	490	292

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination