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Study Information
Study Attribute Value
CEBS Accession Number: 002-02354-0006-0000-2
Chemical Name: Lead(2+) acetate
CASRN: 301-04-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G09316
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 89 141 46 41
    125r 119 143 38
    111 105 119 36
    118 106 150 42
Test Chemical Lead(2+) acetate 
282         
 109 97 129 40
    127r 121 116 30
    123r 123 120 33
  
332         
 104 73 133 42 37
    136r 125 90 22
    115 90 107 31
  
391         
 87 80 110 42 38
    116 93 127 37
    116 102 122 35
  
460         
 118 87 147 42 43
    126r 92 144 38
    116 94 153 44
  
541         
 112 66 189 57 54
    114 69 209 61
    101 65 132 44
  
637         
 105 45 180 57 55
    104 36 168 54
    92 38 152 55
  
750         
 74 8 154 69 63*
    83 12 140 57
    80 9 152 63
Positive Control Ethyl Methane Sulfonate 
500         
 67 43 1135 566 534*
    68# 44 1050 517
    69 45 1083 521

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 103 81 37 45
    83 108 115 46
    72 89 89 41
    81 100 138 57
Test Chemical Lead(2+) acetate 
42         
 80# 157 129 54 51
    78 138 113 48
  
52         
 80 163 97 41 51
    81 151 152 62
  
66         
 81 94 147 61 54
    65 62 92 47
  
82         
 85 74 173 68 67
    80# 96 157.5 66
  
102         
 61 61 102 56 49
    82 80 102 42
  
128         
 70 67 105 50 63
    79 81 183 77
Positive Control 3-Methylcholanthrene 
5         
 73 79 411 187 204*
    67 72 452 227
    67 70 395 197

Non-Induced S9 Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 101 135 55 52
    91 108 129 47
    81 92 148 61
    81 100 107 44
Test Chemical Lead(2+) acetate 
66         
 78 69 198 85 81*
    62 47 185 99
    77 56 134 58
  
78         
 78 76 193 83 66
    84 78 118 47
    65 50 132 68
  
91         
 73 69 145 67 70
    66 57 138 70
    65 61 144 74
  
107         
 70 64 138 66 61
    72 76 122 57
  
126         
 72 69 101 47 45
    87 87 116 44
    82 85 106 43
  
149         
 71 64 149 70 54
    81 87 138 57
    85 93 88 35
  
175         
 73 74 78 36 34
    86 88 90 35
    72 79 66 31
Positive Control 3-Methylcholanthrene 
5         
 62 67 385 206 208*
    71 65 444 209

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75 105 213 94 92
    83 95 224 90
Test Chemical Lead(2+) acetate 
83.9       
 76r 76 234 102
  
105         
 73 77 272 124 121
    72 72 256 118
  
131         
 73 68 256 117 122
    85 96 324 127
  
164         
 81 94 316 131 120
    86 75 283 110
  
205         
 48 53 250 172 150*
    74 73 284 129
  
256         
 86 74 340 132 122
    107 81 361 113
Positive Control 3-Methylcholanthrene 
5         
 20 8 267 441 503*
    18 6 311 565

Induced S9 Trial: 2 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 99 368 141 144
    81 101 358 147
Test Chemical Lead(2+) acetate 
83.9       
 97 118 322 110 107
    101 119 317 104
  
105         
 109 139 340 104
    120r 144 353 98
  
131         
 103 121 300 97
    126r 143 331 88
  
164         
 87 26 340 131 118
    95 74 297 104
  
205         
 77 39 439 189 170
    89 33 405 151
  
256         
  
320         
  
400         
   
Positive Control 3-Methylcholanthrene 
5         
 30 13 480 530 500*
    33 16 470 470

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 98 142 66 63
    94 118 197 70
    75 84 118 53
Test Chemical Lead(2+) acetate 
64.6       
 87 87 266 102 100*
    77 122 226 98
  
71.7       
 83 81 217 87 80
    87 103 188 72
  
79.7       
 96 99 207 72 73
    90 109 209 78
    88 86 182 69
  
88.6       
 89 105 218 82 85
    94 114 242 86
    76 85 195 86
  
98.4       
 95r 106 223 79
  
121.5       
 76r 95 127 56
Positive Control 3-Methylcholanthrene 
5         
 42 24 464 365 377*
    41 23 497 407
    44 21 471 358

Induced S9 Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 110 164 67 65
    93 109 179 64
    82 86 175 71
    92 95 152 55
Test Chemical Lead(2+) acetate 
53.8       
 97 88 219 75 83
    99 100 271 91
  
59.8       
 108 86 193 60 69
    118 109 241 68
    106 93 257 81
  
66.4       
 90 87 163 60 66
    106 112 228 72
    112 114 225 67
  
73.8       
 101 94 256 84 71
    113 113 231 68
    118 122 216 61
  
82         
 109 131 249 76 69
    135r 132 333 82
    107 113 194 61
  
91.1       
 92 104 220 80 77
    95 115 219 77
    117 120 259 74
  
101.2       
 98 122 179 61 66
    114 88 217 64
    92 74 202 73
  
112.5       
 99 92 209 70 79
    90 99 255 94
    109 99 236 72
  
125         
 91 90 242 88 88
    100 108 270 90
    92 112 238 86
Positive Control 3-Methylcholanthrene 
5         
 63 38 561 296 316*
    49 26 524 359
    56 32 490 292

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination