Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02354-0007-0000-3
Chemical Name:	Lead(2+) acetate
CASRN:	301-04-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G76432
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	77	100	51	22	23
Vehicle Control			65	90	48	25	23
Test Chemical	Lead(2+) acetate	156	85	117	78	31	31
			79	87	74	31	31
		313	84	75	67	27	35
			58	65	76	43	35
		625	80	24	125	52	46*
			67	14	79	39	46*
		1250

Positive Control	Ethyl Methane Sulfonate	500	49	56	480	330	321*
Positive Control			53	54	494	313	321*
Negative Control	Negative (Not Specified)	0	75	109	58	26	29*
Negative Control			67	101	66	33	29*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	84	45	22	21
Vehicle Control			91	98	56	21	21
Test Chemical	Lead(2+) acetate	100	77	103	48	21	22
			66	95	48	24	22
		200	84	105	40	16	20
			84	83	63	25	20
		400	85	71	65	25	26
			69	55	54	26	26
		600	60	13	67	38	34
			82	28	75	30	34
		800

Positive Control	Ethyl Methane Sulfonate	500	47	30	990	700	620*
Positive Control			58	37	942	540	620*
Negative Control	Negative (Not Specified)	0	98	110	42	14	17*
Negative Control			80	108	45	19	17*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	109	114	107	33	34
Vehicle Control			91	107	98	36	34
Test Chemical	Lead(2+) acetate	156	92	90	94	34	36
			113	99	126	37	36
		313	108r	88	115	35
			126r	103	113	30
		625	105	75	109	35	44
			108	83	172	53	44
		1250	77	15	335	145	129*
			66	15	224	113	129*
		2500

Positive Control	3-Methylcholanthrene	5	83	42	497	201	222*
Positive Control			68	43	497	243	222*
Negative Control	Negative (Not Specified)	0	106	100	112	35	40*
Negative Control			82	79	110	45	40*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0
Vehicle Control			71#	115	82	38
Test Chemical	Lead(2+) acetate	313	79	50	103	44	34
			91	55	64	23	34
		625	77	94	68	30	35
			80	106	98	41	35
		900	66	47	139	70	64
			84	100	143	57	64
		1250	81	44	150	62	70*
			62	39	145	78	70*
		1500	65	25	336	172	186*
			49	14	296	201	186*
		1800

Positive Control	3-Methylcholanthrene	5	63#	42	477	254	212*
Positive Control			68	39	348	170	212*
Negative Control	Negative (Not Specified)	0	57	91	60	35	38*
Negative Control			63	94	77	41	38*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	84	91	178	71	70
			73#	106	173	80
			85	112	173	68
			108	90	198	61
Test Chemical	Lead(2+) acetate	400	82	45	222	90	80
			94	85	256	90
			85	100	150	59
		800	81	70	327	135	150*
			81	58	421	173
			67	64	286	143
		1000	61	32	521	285	270*
			70	44	486	232
			55	38	486	294
		1400					355*
			28	6	333	394
			39	13	367	316
		1500					312*
			25	4	209	281
			33	5	342	344
		1600


Positive Control	3-Methylcholanthrene	5	37	13	525	469	496*
			45	16	624	466
			38	12	635	552

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination