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Study Information
Study Attribute Value
CEBS Accession Number: 002-02354-0007-0000-3
Chemical Name: Lead(2+) acetate
CASRN: 301-04-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G76432
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 77 100 51 22 23
    65 90 48 25
Test Chemical Lead(2+) acetate 
156         
 85 117 78 31 31
    79 87 74 31
  
313         
 84 75 67 27 35
    58 65 76 43
  
625         
 80 24 125 52 46*
    67 14 79 39
  
1250         
   
Positive Control Ethyl Methane Sulfonate 
500         
 49 56 480 330 321*
    53 54 494 313
Negative Control Negative (Not Specified) 
 0         
 75 109 58 26 29*
    67 101 66 33

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 68 84 45 22 21
    91 98 56 21
Test Chemical Lead(2+) acetate 
100         
 77 103 48 21 22
    66 95 48 24
  
200         
 84 105 40 16 20
    84 83 63 25
  
400         
 85 71 65 25 26
    69 55 54 26
  
600         
 60 13 67 38 34
    82 28 75 30
  
800         
   
Positive Control Ethyl Methane Sulfonate 
500         
 47 30 990 700 620*
    58 37 942 540
Negative Control Negative (Not Specified) 
 0         
 98 110 42 14 17*
    80 108 45 19

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 109 114 107 33 34
    91 107 98 36
Test Chemical Lead(2+) acetate 
156         
 92 90 94 34 36
    113 99 126 37
  
313         
 108r 88 115 35
    126r 103 113 30
  
625         
 105 75 109 35 44
    108 83 172 53
  
1250         
 77 15 335 145 129*
    66 15 224 113
  
2500         
   
Positive Control 3-Methylcholanthrene 
5         
 83 42 497 201 222*
    68 43 497 243
Negative Control Negative (Not Specified) 
 0         
 106 100 112 35 40*
    82 79 110 45

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
    71# 115 82 38
Test Chemical Lead(2+) acetate 
313         
 79 50 103 44 34
    91 55 64 23
  
625         
 77 94 68 30 35
    80 106 98 41
  
900         
 66 47 139 70 64
    84 100 143 57
  
1250         
 81 44 150 62 70*
    62 39 145 78
  
1500         
 65 25 336 172 186*
    49 14 296 201
  
1800         
   
Positive Control 3-Methylcholanthrene 
5         
 63# 42 477 254 212*
    68 39 348 170
Negative Control Negative (Not Specified) 
 0         
 57 91 60 35 38*
    63 94 77 41

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 84 91 178 71 70
    73# 106 173 80
    85 112 173 68
    108 90 198 61
Test Chemical Lead(2+) acetate 
400         
 82 45 222 90 80
    94 85 256 90
    85 100 150 59
  
800         
 81 70 327 135 150*
    81 58 421 173
    67 64 286 143
  
1000         
 61 32 521 285 270*
    70 44 486 232
    55 38 486 294
  
1400         
 355*
    28 6 333 394
    39 13 367 316
  
1500         
 312*
    25 4 209 281
    33 5 342 344
  
1600         
   
   
Positive Control 3-Methylcholanthrene 
5         
 37 13 525 469 496*
    45 16 624 466
    38 12 635 552

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination