Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02366-0008-0000-7
Chemical Name:	Lindane
CASRN:	58-89-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	849017
Study Result:	Equivocal

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	90	123	54	20	26
			70	102	66	31
			66	73	61	31
			75	102	47	21
Test Chemical	Lindane	32	51	78	42	27	22
			79	85	39	16	22
		40	65	61	33	17	16
			64	66	28	15	16
		48	60	25	48	27	19
			59	13	22	12	19
		56	64	27	58	30	26
			69	21	46	22	26
		64	71	29	48	23	32
			59	50	75	42	32
Positive Control	Methyl Methane Sulfonate	15	28	29	195	234	204*
Positive Control			37	30	193	174	204*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	73	112	57	26	35
			66	86	69	35
			72	92	85	39
			61	110	72	39
Test Chemical	Lindane	20	70	105	52	25	25
			74	114	55	25	25
		30	65	96	61	32	31
			92	87	85	31	31
		40	66	64	98	50	49
			76	47	108	48	49
		50	49	6	89	61	56
			49	8	76	52	56
		60

Positive Control	Methyl Methane Sulfonate	15	41	41	198	162	172*
Positive Control			55	48	304	183	172*

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	89	106	46	17	16
			68	87	33	16
			77	107	27	12
			68	100	39	19
Test Chemical	Lindane	30	66	78	45	23	21
			43	56	24	19	21
		35	65	72	43	22	20
			66	61	36	18	20
		40	72	52	33	15	29*
			56	47	72	43	29*
		45	69	24	40	19	22
			65	25	49	25	22
		50	60	12	53	30	27
			77	10	56	24	27
		55

Positive Control	Methyl Methane Sulfonate	15	34	23	109	106	99*
Positive Control			45	26	124	92	99*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	93	81	55	20	31
			90	101	73	27
			89	106	79	30
			75	113	104	46
Test Chemical	Lindane	30	60	46	72	40	40
			66	59	78	40	40
		40	70	42	72	34	32
			67	48	58	29	32
		50	81	49	109	45	52*
			50	34	89	59	52*
		60	70#	26	71	34	42
			72	33	108	50	42
		70	66	22	62	31	32
			84#	17	81	32	32
Positive Control	3-Methylcholanthrene	2.5	36	14	418	391	378*
Positive Control			44	21	481	364	378*

Induced S9 Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	75	98	32	14	25
			65	77	54	28
			84	121	93	37
			61	104	42	23
Test Chemical	Lindane	30	75	79	40	18	31
			66	72	86	43	31
		40	37	57	35	32	29
			63	84	51	27	29
		50	81	86	74	31	30
			59#	80	51	29	30
		60	60	57	55	31	41
			55	45	86	52	41
		70	64	17	129	68	61*
			65	64	107	55	61*
Positive Control	3-Methylcholanthrene	2.5	23	3	123	181	212*
Positive Control			18	4	128	244	212*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	80	107	82	34	37
			72	124	72	33
			71	77	92	43
			70	91	77	37
Test Chemical	Lindane	30	74	67	118	53	54
			77	54	127	55	54
		40	78	61	149	64	61*
			84	63	145	58	61*
		50	78	55	111	47	52
			102	54	175	57	52
		60	77	16	148	64	64*
			71	14	138	65	64*
		70	62	14	101	55	72*
			51	7	136	89	72*
		80
		90

Positive Control	3-Methylcholanthrene	2.5	36	22	619	576	507*
Positive Control			40	27	524	438	507*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination