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Study Information
Study Attribute Value
CEBS Accession Number: 002-02366-0008-0000-7
Chemical Name: Lindane
CASRN: 58-89-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 849017
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 90 123 54 20 26
    70 102 66 31
    66 73 61 31
    75 102 47 21
Test Chemical Lindane 
32         
 51 78 42 27 22
    79 85 39 16
  
40         
 65 61 33 17 16
    64 66 28 15
  
48         
 60 25 48 27 19
    59 13 22 12
  
56         
 64 27 58 30 26
    69 21 46 22
  
64         
 71 29 48 23 32
    59 50 75 42
Positive Control Methyl Methane Sulfonate 
15         
 28 29 195 234 204*
    37 30 193 174

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 73 112 57 26 35
    66 86 69 35
    72 92 85 39
    61 110 72 39
Test Chemical Lindane 
20         
 70 105 52 25 25
    74 114 55 25
  
30         
 65 96 61 32 31
    92 87 85 31
  
40         
 66 64 98 50 49
    76 47 108 48
  
50         
 49 6 89 61 56
    49 8 76 52
  
60         
   
Positive Control Methyl Methane Sulfonate 
15         
 41 41 198 162 172*
    55 48 304 183

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 89 106 46 17 16
    68 87 33 16
    77 107 27 12
    68 100 39 19
Test Chemical Lindane 
30         
 66 78 45 23 21
    43 56 24 19
  
35         
 65 72 43 22 20
    66 61 36 18
  
40         
 72 52 33 15 29*
    56 47 72 43
  
45         
 69 24 40 19 22
    65 25 49 25
  
50         
 60 12 53 30 27
    77 10 56 24
  
55         
   
Positive Control Methyl Methane Sulfonate 
15         
 34 23 109 106 99*
    45 26 124 92

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 93 81 55 20 31
    90 101 73 27
    89 106 79 30
    75 113 104 46
Test Chemical Lindane 
30         
 60 46 72 40 40
    66 59 78 40
  
40         
 70 42 72 34 32
    67 48 58 29
  
50         
 81 49 109 45 52*
    50 34 89 59
  
60         
 70# 26 71 34 42
    72 33 108 50
  
70         
 66 22 62 31 32
    84# 17 81 32
Positive Control 3-Methylcholanthrene 
2.5       
 36 14 418 391 378*
    44 21 481 364

Induced S9 Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 75 98 32 14 25
    65 77 54 28
    84 121 93 37
    61 104 42 23
Test Chemical Lindane 
30         
 75 79 40 18 31
    66 72 86 43
  
40         
 37 57 35 32 29
    63 84 51 27
  
50         
 81 86 74 31 30
    59# 80 51 29
  
60         
 60 57 55 31 41
    55 45 86 52
  
70         
 64 17 129 68 61*
    65 64 107 55
Positive Control 3-Methylcholanthrene 
2.5       
 23 3 123 181 212*
    18 4 128 244

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 80 107 82 34 37
    72 124 72 33
    71 77 92 43
    70 91 77 37
Test Chemical Lindane 
30         
 74 67 118 53 54
    77 54 127 55
  
40         
 78 61 149 64 61*
    84 63 145 58
  
50         
 78 55 111 47 52
    102 54 175 57
  
60         
 77 16 148 64 64*
    71 14 138 65
  
70         
 62 14 101 55 72*
    51 7 136 89
  
80         
  
90         
   
Positive Control 3-Methylcholanthrene 
2.5       
 36 22 619 576 507*
    40 27 524 438

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination