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Study Information
Study Attribute Value
CEBS Accession Number: 002-02373-0001-0000-8
Chemical Name: Lithocholic acid
CASRN: 434-13-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 963393_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 09/07/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 336 0.322 6.72 26.0 0.000
Negative (Not Specified) 
0         
 50 1047 313 0.299 6.26 26.0 -7.023
Test Chemical Lithocholic acid 
1.6       
 50 1040 307 0.295 6.14 26.0 -8.192 
5         
 50 1036 328 0.317 6.56 26.0 -1.533 
16         
 50 1042 383 0.368 7.66 26.0 14.316 
50         
 50 1042 339 0.325 6.78 26.0 1.183 
160         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 0 0 0 0.000 0.00 0.0 N/A
Trend: 1.435        
Probability: 0.076        

Trial #: 2    Activation: No Activation    Date: 11/21/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1044 399 0.382 7.98 26.0 -24.073
Dimethyl Sulfoxide 
0         
 50 1043 525 0.503 10.50 26.0 0.000
Test Chemical Lithocholic acid 
1         
 50 1016 452 0.445 9.04 26.0 -11.617 
5         
 50 1037 478 0.461 9.56 26.0 -8.426 
10         
 50 1020 488 0.478 9.76 26.0 -4.952 
25         
 50 1031 418 0.405 8.36 26.0 -19.454 
50         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1036 1515 1.462 30.30 26.0 190.521
Trend: -2.321        
Probability: 0.990        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/07/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 336 0.321 6.72 26.0 0.000
Negative (Not Specified) 
0         
 50 1044 375 0.359 7.50 26.0 11.928
Test Chemical Lithocholic acid 
 0.5       
 50 1043 338 0.324 6.76 26.0 0.981 
1.6       
 50 1043 367 0.352 7.34 26.0 9.645 
5         
 50 1040 355 0.341 7.10 26.0 6.366 
16         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
1.5       
 50 1044 1278 1.224 25.56 26.0 281.450
Cyclophosphamide 
1.5       
 50 1044 1375 1.317 27.50 26.0 310.402
Trend: 1.117        
Probability: 0.132        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 11/21/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1037 411 0.396 8.22 26.0 1.915
Dimethyl Sulfoxide 
0         
 50 1044 406 0.389 8.12 26.0 0.000
Test Chemical Lithocholic acid 
 0.5       
 50 1040 424 0.408 8.48 26.0 4.835 
1         
 50 1044 423 0.405 8.46 26.0 4.187 
2.5       
 50 1038 433 0.417 8.66 26.0 7.267 
5         
 50 1025 467 0.456 9.34 26.0 17.157 
10         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
2         
 50 1049 2900 2.765 58.00 26.0 610.881
Trend: 2.248        
Probability: 0.012