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Study Information
Study Attribute Value
CEBS Accession Number: 002-02373-0008-0000-5
Chemical Name: Lithocholic acid
CASRN: 434-13-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 244935
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 97 60 27 32
    81 115 64 26
    60 83 74 41
    72 105 67 31
Test Chemical Lithocholic acid 
6.25      
 88 116 63 24 26
    87 112 75 29
  
12.5       
 73 105 61 28 26
    79 115 58 24
  
25         
 87 97 68 26 26
    87 95 70 27
  
50         
 86 19 148 58 55*
    82 18 126 51
  
100         
   
Positive Control Methyl Methane Sulfonate 
15         
 34 28 129 128 112*
    43 36 124 97
Methyl Methane Sulfonate 
250         
 81 91 374 154 155*
    79 89 369 157

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 69 116 73 35 43
    54 82 90 56
    49r 80 70 48
    58 102 64 37
Test Chemical Lithocholic acid 
5         
 75 128 81 36 27
    78 117 41 17
  
10         
 61 108 45 24 22
    69 91 40 19
  
20         
 66 105 43 22 22
    77 116 51 22
  
40         
 83 119 76 30
  
60         
 86 47 60 23 25
    61 43 49 27
  
80         
   
Positive Control Methyl Methane Sulfonate 
15         
 49 45 96 65 79*
    41 37 116 94
Methyl Methane Sulfonate 
250         
 69 104 470 228 218*
    62 107 390 209

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 66 100 58 29 29
    77 96 97 42
    80 113 75 31
    63 91 24 13
Test Chemical Lithocholic acid 
20         
 72 87 70 32 33
    80 78 81 34
  
40         
 65 64 91 47 44
    66 56 80 41
  
60         
 25 5 122 162 145*
    27 5 104 129
  
70         
   
Positive Control Methyl Methane Sulfonate 
15         
 30 22 78 86 88*
    38 27 103 90
Methyl Methane Sulfonate 
250         
 46 45 211 153 144*
    53 61 216 135

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 76 96 71 31 35
    97 97 118 41
    91 113 85 31
    77 94 90 39
Test Chemical Lithocholic acid 
20         
 81 103 100 41 48
    74 88 123 56
  
30         
 85 95 113 44 50
    76 85 125 55
  
40         
 78 58 121 52 59*
    64 47 128 66
  
50         
 60 8 142 79 69*
    77 11 136 59
  
60         
   
Positive Control Methyl Methane Sulfonate 
15         
 29 18 181 210 198*
    27 19 150 185

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination