Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02373-0008-0000-5
Chemical Name:	Lithocholic acid
CASRN:	434-13-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	244935
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	97	60	27	32
			81	115	64	26
			60	83	74	41
			72	105	67	31
Test Chemical	Lithocholic acid	6.25	88	116	63	24	26
			87	112	75	29	26
		12.5	73	105	61	28	26
			79	115	58	24	26
		25	87	97	68	26	26
			87	95	70	27	26
		50	86	19	148	58	55*
			82	18	126	51	55*
		100

Positive Control	Methyl Methane Sulfonate	15	34	28	129	128	112*
			43	36	124	97	112*
	Methyl Methane Sulfonate	250	81	91	374	154	155*
			79	89	369	157	155*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	116	73	35	43
			54	82	90	56
			49r	80	70	48
			58	102	64	37
Test Chemical	Lithocholic acid	5	75	128	81	36	27
			78	117	41	17	27
		10	61	108	45	24	22
			69	91	40	19	22
		20	66	105	43	22	22
			77	116	51	22	22
		40	83	119	76	30
		60	86	47	60	23	25
			61	43	49	27	25
		80

Positive Control	Methyl Methane Sulfonate	15	49	45	96	65	79*
			41	37	116	94	79*
	Methyl Methane Sulfonate	250	69	104	470	228	218*
			62	107	390	209	218*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	100	58	29	29
			77	96	97	42
			80	113	75	31
			63	91	24	13
Test Chemical	Lithocholic acid	20	72	87	70	32	33
			80	78	81	34	33
		40	65	64	91	47	44
			66	56	80	41	44
		60	25	5	122	162	145*
			27	5	104	129	145*
		70

Positive Control	Methyl Methane Sulfonate	15	30	22	78	86	88*
			38	27	103	90	88*
	Methyl Methane Sulfonate	250	46	45	211	153	144*
			53	61	216	135	144*

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	96	71	31	35
			97	97	118	41
			91	113	85	31
			77	94	90	39
Test Chemical	Lithocholic acid	20	81	103	100	41	48
			74	88	123	56	48
		30	85	95	113	44	50
			76	85	125	55	50
		40	78	58	121	52	59*
			64	47	128	66	59*
		50	60	8	142	79	69*
			77	11	136	59	69*
		60

Positive Control	Methyl Methane Sulfonate	15	29	18	181	210	198*
Positive Control			27	19	150	185	198*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination