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Study Information
Study Attribute Value
CEBS Accession Number: 002-02373-0009-0000-6
Chemical Name: Lithocholic acid
CASRN: 434-13-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G56739
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 73 55 26 28
    83 98 68 27
    114 131 88 26
    108 98 110 34
Test Chemical Lithocholic acid 
42         
 64 106 81 42 36
    77 92 80 34
    69 91 63 30
  
53         
 82 108 47 19 28
    90 94 94 35
    95 115 87 30
  
66         
 80 76 64 27 29
    73 70 71 32
    92 114 73 26
  
82         
 71 58 90 42 39
    83 66 89 36
    81 43 97 40
  
91         
 71 32 157 73 70*
    73 22 123 57
    66 26 156 79
  
101         
 63 7 250 133 154*
    64 6 386 201
    69 8 263 127
  
113         
   
   
Positive Control Ethyl Methane Sulfonate 
500         
 58 39 1036 594 530*
    80 46 1190 497
    79 43 1189 500

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 100 113 51 46
    89 113 117 44
    83 101 126 51
    79 87 97 41
Test Chemical Lithocholic acid 
61.1       
 92 144 345 126 118*
    94 118 309 110
  
67.9       
 80 127 215 90 95*
    74 99 224 101
  
75.5       
 91 94 209 77 79*
    92 106 224 81
  
83.8       
 87 50 296 113
  
93.2       
 73 11 891 405 356*
    69 13 634 308
  
103.5       
 58 4 1614 933 1117*
    46 3 1790 1302
  
115         
   
Positive Control Ethyl Methane Sulfonate 
500         
 43 32 1008 778 761*
    43 36 958 743

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 81 260 109 84
    100 105 277 92
    108 110 237 73
    95 104 179 63
Test Chemical Lithocholic acid 
25         
 76 96 422 186 171*
    83 85 402 161
    77 86 378 165
  
31         
 74 77 364 164 149*
    86 95 335 130
    83 94 379 153
  
39         
 82 90 340 138 119
    95 106 253 89
    79 91 305 129
  
49         
 86 89 351 137 127*
    78 86 274 117
    88 83 341 129
  
61         
 77 38 277 119 109
    87 25 289 111
    79 33 229 97
  
77         
 252*
    51 4 411 267
    60 4 423 237
  
96         
   
   
Positive Control 3-Methylcholanthrene 
5         
 79 72 613 259 273*
    57 34 621 362
    95 86 562 198

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 103 204 93 93
    74 94 218 99
    78 111 181 77
    65 93 200 102
Test Chemical Lithocholic acid 
50         
 66 96 185 93 95
    70 93 203 97
  
56         
 66 94 144 73 72
    87 123 184 71
  
62         
 67# 78 217 108 109
    60 79 196 109
  
65         
 64 68 219 114 105
    71 86 207 97
  
69         
 73 69 232 106 107
    60 53 194 109
  
72         
 73# 43 273 125 116
    68 37 219 108
  
76         
 47 13 173 124 142
    50 18 242 161
Positive Control 3-Methylcholanthrene 
5         
 43 32 527 410 380*
    50 39 503 336
    44 33 517 395

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 112 185 84 90
    73 93 175 80
    70 103 213 102
    68# 92 192 94
Test Chemical Lithocholic acid 
56         
 69 73 182 88 92
    71 77 185 87
    73 65 221 101
  
62         
 65 46 236 121 102
    65 55 197 101
    64 52 162 85
  
69         
 58 31 224 128 128
    64 30 245 129
    53 26 204 127
  
72         
 62 25 165 89 111
    58 23 248 143
    63 29 194 103
  
76         
 45 13 154 115 116
    41 14 159 129
    56 17 173 103
  
80         
 50 7 113 75 93
    63 15 189 100
    57 10 176 104
Positive Control 3-Methylcholanthrene 
5         
 44 41 644 484 494*
    40 34 599 503

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination