Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02373-0009-0000-6
Chemical Name:	Lithocholic acid
CASRN:	434-13-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G56739
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	73	55	26	28
			83	98	68	27
			114	131	88	26
			108	98	110	34
Test Chemical	Lithocholic acid	42	64	106	81	42	36
			77	92	80	34
			69	91	63	30
		53	82	108	47	19	28
			90	94	94	35
			95	115	87	30
		66	80	76	64	27	29
			73	70	71	32
			92	114	73	26
		82	71	58	90	42	39
			83	66	89	36
			81	43	97	40
		91	71	32	157	73	70*
			73	22	123	57
			66	26	156	79
		101	63	7	250	133	154*
			64	6	386	201
			69	8	263	127
		113


Positive Control	Ethyl Methane Sulfonate	500	58	39	1036	594	530*
			80	46	1190	497
			79	43	1189	500

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	100	113	51	46
			89	113	117	44
			83	101	126	51
			79	87	97	41
Test Chemical	Lithocholic acid	61.1	92	144	345	126	118*
			94	118	309	110	118*
		67.9	80	127	215	90	95*
			74	99	224	101	95*
		75.5	91	94	209	77	79*
			92	106	224	81	79*
		83.8	87	50	296	113
		93.2	73	11	891	405	356*
			69	13	634	308	356*
		103.5	58	4	1614	933	1117*
			46	3	1790	1302	1117*
		115

Positive Control	Ethyl Methane Sulfonate	500	43	32	1008	778	761*
Positive Control			43	36	958	743	761*

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	81	260	109	84
			100	105	277	92
			108	110	237	73
			95	104	179	63
Test Chemical	Lithocholic acid	25	76	96	422	186	171*
			83	85	402	161
			77	86	378	165
		31	74	77	364	164	149*
			86	95	335	130
			83	94	379	153
		39	82	90	340	138	119
			95	106	253	89
			79	91	305	129
		49	86	89	351	137	127*
			78	86	274	117
			88	83	341	129
		61	77	38	277	119	109
			87	25	289	111
			79	33	229	97
		77					252*
			51	4	411	267
			60	4	423	237
		96


Positive Control	3-Methylcholanthrene	5	79	72	613	259	273*
			57	34	621	362
			95	86	562	198

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	103	204	93	93
			74	94	218	99
			78	111	181	77
			65	93	200	102
Test Chemical	Lithocholic acid	50	66	96	185	93	95
			70	93	203	97	95
		56	66	94	144	73	72
			87	123	184	71	72
		62	67#	78	217	108	109
			60	79	196	109	109
		65	64	68	219	114	105
			71	86	207	97	105
		69	73	69	232	106	107
			60	53	194	109	107
		72	73#	43	273	125	116
			68	37	219	108	116
		76	47	13	173	124	142
			50	18	242	161	142
Positive Control	3-Methylcholanthrene	5	43	32	527	410	380*
			50	39	503	336
			44	33	517	395

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	112	185	84	90
			73	93	175	80
			70	103	213	102
			68#	92	192	94
Test Chemical	Lithocholic acid	56	69	73	182	88	92
			71	77	185	87
			73	65	221	101
		62	65	46	236	121	102
			65	55	197	101
			64	52	162	85
		69	58	31	224	128	128
			64	30	245	129
			53	26	204	127
		72	62	25	165	89	111
			58	23	248	143
			63	29	194	103
		76	45	13	154	115	116
			41	14	159	129
			56	17	173	103
		80	50	7	113	75	93
			63	15	189	100
			57	10	176	104
Positive Control	3-Methylcholanthrene	5	44	41	644	484	494*
Positive Control			40	34	599	503	494*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination