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Study Information
Study Attribute Value
CEBS Accession Number: 002-02380-0019-0000-5
Chemical Name: Malaoxon
CASRN: 1634-78-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 566519
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 106 122 66 21 26
    92# 96 73 26
    70 82 67 32
Test Chemical Malaoxon 
25         
 102 129 61 20 23
    112# 128 99 30
    118# 144 66 19
  
50         
 97# 97 75 26 23
    110 137 68 21
    105 133 72 23
  
100         
 116# 84 121 35 32
    94 90 100 36
    90 78 72 27
  
200         
 86 17 200 77 66*
    75 25 133 59
    85 23 154 60
  
300         
   
  
400         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 37 38 507 459 358*
    51 47 574 374
    63 69 451 240

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 107 107 154 48 44
    108 77 154 47
    108 96 142 44
    115 119 127 37
Test Chemical Malaoxon 
12.5       
 108 94 127 39 39
    106 107 121 38
    115 101 133 39
  
25         
 114 94 85 25 29
    105# 121 75 24
    101 101 113 37
  
50         
 106 71 189 60 56
    97 48 172 59
    112 55 164 49
  
75         
 116 42 185 53 59
    114 47 207 61
    101 51 193 64
  
100         
 106 37 210 66 68*
    100 47 196 65
    113 33 250 74
  
150         
 98 7 415 141 119*
    88 21 283 107
    106 19 346 109
  
200         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 99 43 945 318 302*
    116 69 973 280
    106 75 978 308

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 98 84 140 48 52
    95 95 174 61
    92 110 132 48
    81 111 122 50
Test Chemical Malaoxon 
75         
 107 84 220 69 69
    100 91 205 68
    89 77 190 71
  
100         
 115 93 231 67 64
    95 89 179 63
    117 89 214 61
  
150         
 106 69 220 69 70
    117 76 251 72
    120r 79 241 67
  
200         
 120r 99 253 70 93*
    118 46 301 85
    101 46 307 101
  
250         
 112 41 207 62
    128r 29 425 111
    126r 34 309 82
  
300         
 123r 10 209 57 54
    114 8 141 41
    109 12 217 66
Positive Control 3-Methylcholanthrene 
2.5       
 90# 56 363 134 149*
    88 62 531 202
    94 70 316 112

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 108 99 130 40 48
    120 104 149 42
    111 94 177 53
    93 103 154 55
Test Chemical Malaoxon 
50         
 107 94 167 52 62
    107 91 249 78
    114 109 193 57
  
75         
 109 95 184 56 64
    117 94 199 57
    82# 84 191 78
  
100         
 86 89 150 58 61
    105# 89 169 54
    110 115 237 72
  
150         
 105 77 253 80 56
    115 60 156 45
    113 94 145 43
  
200         
 58
    112 53 189 56
    110 69 195 59
  
250         
 120 72 252 70 68
    105 28 210 67
   
  
300         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 117# 63 986 280 283*
    113 109 793 234
    88 54 890 336

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 112 81 136 40 49
    109 114 198 60
    107 97 156 49
    112 108 159 47
Test Chemical Malaoxon 
50         
 117 90 172 49 43
    109 146 127 39
    107 111 137 43
  
75         
 89 82 180 68 55
    104 83 159 51
    106 79 145 46
  
100         
 115 107 188 55 48
    117 112 165 47
    120 105 155 43
  
150         
 110 46 150 46 53
    100 43 177 59
    110 49 178 54
  
200         
 92 46 191 69 54
    113 36 135 40
    107 23 170 53
  
300         
 65 4 149 76 78*
    117 28 261 74
    102 21 257 84
Positive Control 3-Methylcholanthrene 
2.5       
 108 43 763 236 278*
    101 50 853 283
    102 50 964 314

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination