Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02380-0019-0000-5
Chemical Name:	Malaoxon
CASRN:	1634-78-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	566519
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	106	122	66	21	26
			92#	96	73	26
			70	82	67	32
Test Chemical	Malaoxon	25	102	129	61	20	23
			112#	128	99	30
			118#	144	66	19
		50	97#	97	75	26	23
			110	137	68	21
			105	133	72	23
		100	116#	84	121	35	32
			94	90	100	36
			90	78	72	27
		200	86	17	200	77	66*
			75	25	133	59
			85	23	154	60
		300

		400


Positive Control	Methyl Methane Sulfonate	5	37	38	507	459	358*
			51	47	574	374
			63	69	451	240

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	107	107	154	48	44
			108	77	154	47
			108	96	142	44
			115	119	127	37
Test Chemical	Malaoxon	12.5	108	94	127	39	39
			106	107	121	38
			115	101	133	39
		25	114	94	85	25	29
			105#	121	75	24
			101	101	113	37
		50	106	71	189	60	56
			97	48	172	59
			112	55	164	49
		75	116	42	185	53	59
			114	47	207	61
			101	51	193	64
		100	106	37	210	66	68*
			100	47	196	65
			113	33	250	74
		150	98	7	415	141	119*
			88	21	283	107
			106	19	346	109
		200


Positive Control	Methyl Methane Sulfonate	5	99	43	945	318	302*
			116	69	973	280
			106	75	978	308

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	98	84	140	48	52
			95	95	174	61
			92	110	132	48
			81	111	122	50
Test Chemical	Malaoxon	75	107	84	220	69	69
			100	91	205	68
			89	77	190	71
		100	115	93	231	67	64
			95	89	179	63
			117	89	214	61
		150	106	69	220	69	70
			117	76	251	72
			120r	79	241	67
		200	120r	99	253	70	93*
			118	46	301	85
			101	46	307	101
		250	112	41	207	62
			128r	29	425	111
			126r	34	309	82
		300	123r	10	209	57	54
			114	8	141	41
			109	12	217	66
Positive Control	3-Methylcholanthrene	2.5	90#	56	363	134	149*
			88	62	531	202
			94	70	316	112

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	108	99	130	40	48
			120	104	149	42
			111	94	177	53
			93	103	154	55
Test Chemical	Malaoxon	50	107	94	167	52	62
			107	91	249	78
			114	109	193	57
		75	109	95	184	56	64
			117	94	199	57
			82#	84	191	78
		100	86	89	150	58	61
			105#	89	169	54
			110	115	237	72
		150	105	77	253	80	56
			115	60	156	45
			113	94	145	43
		200					58
			112	53	189	56
			110	69	195	59
		250	120	72	252	70	68
			105	28	210	67

		300


Positive Control	3-Methylcholanthrene	2.5	117#	63	986	280	283*
			113	109	793	234
			88	54	890	336

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	112	81	136	40	49
			109	114	198	60
			107	97	156	49
			112	108	159	47
Test Chemical	Malaoxon	50	117	90	172	49	43
			109	146	127	39
			107	111	137	43
		75	89	82	180	68	55
			104	83	159	51
			106	79	145	46
		100	115	107	188	55	48
			117	112	165	47
			120	105	155	43
		150	110	46	150	46	53
			100	43	177	59
			110	49	178	54
		200	92	46	191	69	54
			113	36	135	40
			107	23	170	53
		300	65	4	149	76	78*
			117	28	261	74
			102	21	257	84
Positive Control	3-Methylcholanthrene	2.5	108	43	763	236	278*
			101	50	853	283
			102	50	964	314

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination