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Study Information
Study Attribute Value
CEBS Accession Number: 002-02383-0003-0000-1
Chemical Name: Maleic hydrazide
CASRN: 123-33-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 702766_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
No Activation  3  Positive 
No Activation  4  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 12/23/1981    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 409 0.390 8.18 26.0 0.000
Test Chemical Maleic hydrazide 
16         
 50 1034 505 0.488 10.10 26.0 25.263 
50         
 50 1033 544 0.527 10.88 26.0 35.067 
160         
 50 1027 848 0.826 16.96 26.0 111.776 
500         
 50 1043 1799 1.725 35.98 26.0 342.384
Positive Control Mitomycin-C 
 0.01      
 50 1024 2081 2.032 41.62 26.0 421.224
Trend: 34.538        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/26/1981    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1047 386 0.369 7.72 26.0 0.000
Test Chemical Maleic hydrazide 
16         
 50 1046 403 0.385 8.06 26.0 4.504 
50         
 50 1047 495 0.473 9.90 26.0 28.238 
160         
 50 1042 705 0.677 14.10 26.0 83.519 
500         
 50 1041 1740 1.671 34.80 26.0 353.375 
1600         
 0 0 0 0.000 0.00 26.0 N/A 
5000         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1041 1707 1.640 34.14 26.0 344.777
Trend: 34.985        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 01/21/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1033 422 0.409 8.44 26.0 0.000
Test Chemical Maleic hydrazide 
50         
 50 1049 504 0.480 10.08 26.0 17.610 
160         
 50 1032 663 0.642 13.26 26.0 57.261 
500         
 50 1046 1294 1.237 25.88 26.0 202.824 
1000         
 50 1040 1710 1.644 34.20 26.0 302.486 
2000         
 50 1031 2325 2.255 46.50 26.0 452.017 
3000         
 50 1042 2866 2.750 57.32 26.0 573.281 
4000         
 50 1031 3154 3.059 63.08 26.0 648.843
Positive Control Mitomycin-C 
 0.005     
 100 1030 1761 1.710 17.61 26.0 318.514
Trend: 67.645        
Probability: 0.000        

Trial #: 4    Activation: No Activation    Date: 01/21/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1027 380 0.370 7.60 26.0 0.000
Test Chemical Maleic hydrazide 
1000         
 50 1032 396 0.384 7.92 26.0 3.706 
2000         
 50 1037 434 0.419 8.68 26.0 13.109 
3000         
 50 1035 410 0.396 8.20 26.0 7.061 
4000         
 50 1033 416 0.403 8.32 26.0 8.838
Positive Control Mitomycin-C 
 0.005     
 50 1030 1761 1.710 35.22 26.0 362.071
Trend: 1.397        
Probability: 0.081        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/08/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1033 391 0.379 7.82 26.0 0.000
Test Chemical Maleic hydrazide 
500         
 50 1033 394 0.381 7.88 26.0 0.767 
1000         
 50 1047 463 0.442 9.26 26.0 16.831 
2000         
 50 1037 416 0.401 8.32 26.0 5.983 
3000         
 50 1038 436 0.420 8.72 26.0 10.972 
4000         
 50 1039 458 0.441 9.16 26.0 16.459
Positive Control Cyclophosphamide 
1.5       
 50 1040 1584 1.523 31.68 26.0 302.388
Trend: 2.283        
Probability: 0.011        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 01/13/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1040 405 0.389 8.10 26.0 0.000
Test Chemical Maleic hydrazide 
2000         
 50 1027 406 0.395 8.12 26.0 1.516 
3000         
 50 1039 558 0.537 11.16 26.0 37.910 
4000         
 50 1048 714 0.681 14.28 26.0 74.951
Positive Control Cyclophosphamide 
2         
 50 1035 2262 2.186 45.24 26.0 461.217
Trend: 10.304        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 01/21/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1040 511 0.491 10.22 26.0 0.000
Test Chemical Maleic hydrazide 
1000         
 50 1035 465 0.449 9.30 26.0 -8.562 
2000         
 50 1036 460 0.444 9.20 26.0 -9.633 
3000         
 50 1041 467 0.449 9.34 26.0 -8.698 
4000         
 50 1040 495 0.476 9.90 26.0 -3.131
Positive Control Cyclophosphamide 
2         
 50 1049 2344 2.235 46.88 26.0 354.773
Trend: -0.650        
Probability: 0.742