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Study Information
Study Attribute Value
CEBS Accession Number: 002-02393-0010-0000-0
Chemical Name: Melamine
CASRN: 108-78-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 091419
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 101 120 47 51
    96 106 152 53
    95# 88 108 38
    90 105 180 66
Test Chemical Melamine 
10         
 88 74 138 52 49
    78 109 107 46
  
20         
 52 51 99 63 58
    77 93 124 53
  
40         
 83# 86 93 37 38
    93 86 110 39
  
80         
 92 115 115 42 41
    80 98 95 40
  
160         
 83 84 116 47 42
    96 106 105 37
Positive Control Methyl Methane Sulfonate 
15         
 34 21 281 274 287*
    32 20 283 299

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 93 109 81 29 38
    99 105 115 39
    89 93 104 39
    85 93 117 46
Test Chemical Melamine 
80         
 74 95 100 45 43
    91 108 112 41
  
100         
 92 104 148 54 55
    73 83 124 57
  
120         
 85 88 132 52 49
    88 98 122 46
  
140         
 98 97 95 32 42
    79 83 123 52
  
160         
 82 79 133 54 46
    99 90 111 37
Positive Control Methyl Methane Sulfonate 
15         
 47 33 218 154 169*
    42 35 230 185

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 90 128 45 40
    89 84 130 49
    91 112 87 32
    115 114 118 34
Test Chemical Melamine 
80         
 145r 144 132 30
    127r 100 151 40
  
100         
 100 90 164 55 58
    100 86 186 62
  
120         
 75 72 93 41 51
    106 97 191 60
  
140         
 89 92 131 49 48
    99 83 138 46
  
160         
 107 75 190 59 66*
    103 61 222 72
Positive Control 3-Methylcholanthrene 
2.5       
 96 59 579 200 188*
    83 68 438 175

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 109 84 31 30
    94 89 98 35
    112 95 86 26
    90 107 73 27
Test Chemical Melamine 
80         
 100 101 95 32 38
    78 94 103 44
  
100         
 103 98 107 35 32
    100 93 88 29
  
120         
 91 96 111 41 36
    102 91 95 31
  
140         
 82 87 93 38 37
    98 90 105 36
  
160         
 75 89 99 44 39
    107 71 108 34
Positive Control 3-Methylcholanthrene 
2.5       
 62 30 426 231 232*
    55 28 383 232

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination