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Study Information
Study Attribute Value
CEBS Accession Number: 002-02395-0001-0000-2
Chemical Name: Melphalan
CASRN: 148-82-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 283792_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 08/06/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 434 0.414 8.68 26.0 0.000
Test Chemical Melphalan 
 0.01      
 50 1046 589 0.563 11.78 26.0 35.974 
 0.016     
 50 1050 791 0.753 15.82 26.0 81.911 
 0.03      
 50 1049 967 0.922 19.34 26.0 122.599 
 0.05      
 50 1048 1261 1.203 25.22 26.0 190.553
Positive Control Mitomycin-C 
 0.0E-4    
 50 1048 651 0.621 13.02 26.0 50.000
Mitomycin-C 
 0.005     
 10 211 269 1.275 26.90 26.0 207.852
Trend: 22.561        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 08/27/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 447 0.426 8.94 26.0 0.000
Test Chemical Melphalan 
 0.01      
 50 1048 783 0.747 15.66 26.0 75.335 
 0.016     
 50 1047 835 0.798 16.70 26.0 87.158 
 0.03      
 50 1050 1270 1.210 25.40 26.0 183.846 
 0.05      
 50 1053 1688 1.603 33.76 26.0 276.194
Positive Control Mitomycin-C 
 0.0E-4    
 50 1044 606 0.580 12.12 26.0 36.220
Mitomycin-C 
 0.005     
 10 210 230 1.095 23.00 26.0 157.026
Trend: 29.676        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 06/17/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1048 433 0.413 8.66 26.0 0.000
Test Chemical Melphalan 
 0.16      
 5 105 567 5.400 113.40 26.0 1206.975 
 0.5       
 5 106 889 8.387 177.80 26.0 1929.875 
1.6       
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
 0.2       
 50 1048 958 0.914 19.16 26.0 121.247
Cyclophosphamide 
 0.6       
 10 210 334 1.590 33.40 26.0 284.947
Trend: 72.024        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/06/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 444 0.424 8.88 26.0 0.000
Test Chemical Melphalan 
 0.01      
 50 1050 508 0.484 10.16 26.0 13.979 
 0.016     
 50 1048 571 0.545 11.42 26.0 28.358 
 0.03      
 50 1050 649 0.618 12.98 26.0 45.614 
 0.05      
 50 1047 939 0.897 18.78 26.0 111.284
Positive Control Cyclophosphamide 
 0.2       
 50 1049 927 0.884 18.54 26.0 108.187
Cyclophosphamide 
 0.6       
 10 210 301 1.433 30.10 26.0 237.673
Trend: 14.346        
Probability: 0.000