Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02398-0009-0000-3
Chemical Name:	Mercuric chloride
CASRN:	7487-94-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	289120
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	61#	101	103.5	56	46
			68#	92	93	46
			70	104	87	42
			79	103	92	39
Test Chemical	Mercuric chloride	0.1	60#	56	75	42	36
			66	53	60	30	36
		0.2	68	51	141	69	67
			56	47	107	64	67
		0.4	63	19	156	83	84*
			62	23	159	86	84*
		0.8	46	7	305	222	215*
			48	6	299	208	215*
		1.6

Positive Control	Methyl Methane Sulfonate	15	28	22	258	311	285*
Positive Control			24	16	183	260	285*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71#	95	97	46	54
			85#	109	160.5	63
			83	95	129	52
Test Chemical	Mercuric chloride	0.2107	66#	37	184.5	93	85*
			85#	45	196.5	77	85*
		0.316	74	49	140	63	53
			106#	50	135	42	53
		0.4741	69#	21	128	62	53
			103#	33	136.5	44	53
		0.7111	67	13	201	100
		1.067	75#	12	217	96	99*
			73#	10	222	102	99*
		1.6

Positive Control	Methyl Methane Sulfonate	15	20#	20	199.5	333	333*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70#	89	111	53	49
			79#	112	85.5	36
			54#	99	94.5	58
Test Chemical	Mercuric chloride	0.1	56#	65	97.5	59	57
			60#	58	100	56	57
		0.3	59	39	111	62	53
			52#	36	69	44	53
		0.5	61#	20	220.5	121	121*
			60	13	217	121	121*
		0.7	64#	22	207	108
		0.9	65	9	276	141
Positive Control	Methyl Methane Sulfonate	15	17	16	236	467	467*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination