Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02417-0010-0000-7
Chemical Name:	Methapyrilene hydrochloride
CASRN:	135-23-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	860299
Study Result:	Negative

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	69	192	94	73
			105	107	211	67
			100	114	195	65
			93	109	188	67
Test Chemical	Methapyrilene hydrochloride	50	88	77	191	72	76
			90	74	213	79	76
		100	64	59	195	102	100
			78	62	229	98	100
		200	65	37	241	124	121*
			68	42	238	117	121*
		300	87	35	185	71	96
			51	20	183	120	96
		400

Positive Control	Methyl Methane Sulfonate	15	26	16	438	551	406*
Positive Control			47	27	368	262	406*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	94	89	44	46
			82	92	108	44
			71	109	85	40
			65	105	109	56
Test Chemical	Methapyrilene hydrochloride	100	56	64	106	63	53
			65	79	84	43	53
		200	54	51	98	61	58
			55	54	90	55	58
		300	54	39	98	60	61
			49	36	92	62	61
		400

Positive Control	Methyl Methane Sulfonate	15	36	29	130	120	121*
Positive Control			43	33	154	121	121*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	69	100	48	23	24
			76	97	46	20
			79	98	68	29
			77	105	52	23
Test Chemical	Methapyrilene hydrochloride	100	68	98	54	27	30
			93	97	90	32	30
		200	57	70	67	39	35
			72	84	68	31	35
		300	80	84	82	34	39*
			74	67	97	44	39*
		400	97	67	88	30	32
			88	65	90	34	32
		500	99	39	95	32	36
			79	55	95	40	36
Positive Control	3-Methylcholanthrene	2.5	49	26	519	353	355*
Positive Control			55	26	592	357	355*

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	83	105	89	36	42
			75	99	105	47
			79	109	98	41
			74	88	103	46
Test Chemical	Methapyrilene hydrochloride	150	52	54	104	66	42
			53	57	27	17	42
		250	63	45	40	21	22
			67	64	44	22	22
		350	64	38	59	31	36
			65	40	78	40	36
		450	69	33	48	23	27
			63	28	57	30	27
		550	49	5	43	29	28
			63	12	50	26	28
		650

Positive Control	3-Methylcholanthrene	2.5	71	66	381	180	197*
Positive Control			72	53	463	213	197*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination