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Study Information
Study Attribute Value
CEBS Accession Number: 002-02429-0010-0000-0
Chemical Name: Methyl carbamate
CASRN: 598-55-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 089158
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 95 121 108 38 40
    94 83 129 46
    88 96 96 37
Test Chemical Methyl carbamate 
1049         
 73 59 110 50 46
    89 109 110 41
  
1311         
 84 99 126 50
  
1638         
 76 69 96 42 50
    84 78 144 57
  
2048         
 97# 124 159 55 66*
    80 108 186 77
  
2560         
 74 75 113 51 51
    101 116 156 52
  
3200         
 69 50 101 49 46
    76 79 98 43
  
4000         
 82 76 89 36 40
    78 77 104 45
  
5000         
 96 134 114 40 38
    79 50 88 37
Positive Control Ethyl Methane Sulfonate 
500         
 51 42 1079 710 736*
    49 27 984 665
    45 34 1113 834

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 75 105 87 38 46
    76 105 102 45
    74 102 78 35
    69 87 133 64
Test Chemical Methyl carbamate 
512         
 75 114 118 53 49
    86 123 116 45
  
1024         
 75 127 127 57 54
    78 118 119 51
  
2048         
 87 96 82 32 34
    66 101 74 37
  
2560         
 67 97 74 37 43
    61 93 88 48
  
3200         
 70 108 89 42 43
    60 88 79 44
  
4000         
 81 112 135 56 56
    73 92 125 57
  
5000         
 70 93 100 48 45
    78 99 99 42
Positive Control Ethyl Methane Sulfonate 
500         
 25 19 843 1124 1150*
    29 24 921 1065
    23 18 858 1262

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 93 97 54 19 27
    94 106 122 43
    92 100 54 20
    87 98 65 25
Test Chemical Methyl carbamate 
1638         
 82 82 82 34 33
    72 77 70 32
    84 84 83 33
  
2048         
 71 87 90 42 30
    88 95 47 18
    81# 93 75 31
  
2560         
 80 90 53 22 26
    82 89 50 20
    79 82 86 36
  
3200         
 72 78 99 46 26
    79 86 37 16
    97 110 46 16
  
4000         
 101 98 91 30 32
    89 78 82 31
    96 98 99 35
  
5000         
 94# 100 87 31 26
    94 89 61 22
Positive Control Ethyl Methane Sulfonate 
500         
 57 40 929 543 568*
    53 37 938 588
    55 37 938 574

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 80 92 106 44 37
    90 102 89 33
    85 107 84 33
Test Chemical Methyl carbamate 
588         
 74 101 138 62 50
    72 113 83 38
  
840         
 92 118 118 43 50
    87 83 128 49
    73 84 126 58
  
1201         
 84 86 98 39 38
    111 115 114 34
    85 92 101 40
  
1715         
 102 99 110 36 33
    88# 93 83 31
    95 86 88 31
  
2450         
 104 97 90 29 32
    107 103 106 33
    99 96 97 33
  
3500         
 98 92 103 35 34
    93 90 101 36
    106 108 101 32
  
5000         
 95 97 90 31 34
    92 98 114 41
    85 87 75 29
Positive Control 3-Methylcholanthrene 
5         
 82 75 368 150 155*
    75 70 364 162
    81 72 372 153

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 78 109 58 25 26
    79 105 58 24
    71 86 59 28
Test Chemical Methyl carbamate 
2048         
 70# 82 39 19
  
2560         
 54 65 69 42 33
    65 76 46 24
  
3200         
 64 98 36 19 25
    67 110 62 31
  
4000         
 63 81 84 45
  
5000         
 71 83 72 34 25
    72 85 36 17
Positive Control 3-Methylcholanthrene 
5         
 59 54 234 132 132*
    56 53 232 137
    54 63 208 128

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination