Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02429-0010-0000-0
Chemical Name:	Methyl carbamate
CASRN:	598-55-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	089158
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	95	121	108	38	40
			94	83	129	46
			88	96	96	37
Test Chemical	Methyl carbamate	1049	73	59	110	50	46
			89	109	110	41	46
		1311	84	99	126	50
		1638	76	69	96	42	50
			84	78	144	57	50
		2048	97#	124	159	55	66*
			80	108	186	77	66*
		2560	74	75	113	51	51
			101	116	156	52	51
		3200	69	50	101	49	46
			76	79	98	43	46
		4000	82	76	89	36	40
			78	77	104	45	40
		5000	96	134	114	40	38
			79	50	88	37	38
Positive Control	Ethyl Methane Sulfonate	500	51	42	1079	710	736*
			49	27	984	665
			45	34	1113	834

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	75	105	87	38	46
			76	105	102	45
			74	102	78	35
			69	87	133	64
Test Chemical	Methyl carbamate	512	75	114	118	53	49
			86	123	116	45	49
		1024	75	127	127	57	54
			78	118	119	51	54
		2048	87	96	82	32	34
			66	101	74	37	34
		2560	67	97	74	37	43
			61	93	88	48	43
		3200	70	108	89	42	43
			60	88	79	44	43
		4000	81	112	135	56	56
			73	92	125	57	56
		5000	70	93	100	48	45
			78	99	99	42	45
Positive Control	Ethyl Methane Sulfonate	500	25	19	843	1124	1150*
			29	24	921	1065
			23	18	858	1262

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	93	97	54	19	27
			94	106	122	43
			92	100	54	20
			87	98	65	25
Test Chemical	Methyl carbamate	1638	82	82	82	34	33
			72	77	70	32
			84	84	83	33
		2048	71	87	90	42	30
			88	95	47	18
			81#	93	75	31
		2560	80	90	53	22	26
			82	89	50	20
			79	82	86	36
		3200	72	78	99	46	26
			79	86	37	16
			97	110	46	16
		4000	101	98	91	30	32
			89	78	82	31
			96	98	99	35
		5000	94#	100	87	31	26
			94	89	61	22	26
Positive Control	Ethyl Methane Sulfonate	500	57	40	929	543	568*
			53	37	938	588
			55	37	938	574

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	80	92	106	44	37
			90	102	89	33
			85	107	84	33
Test Chemical	Methyl carbamate	588	74	101	138	62	50
			72	113	83	38	50
		840	92	118	118	43	50
			87	83	128	49
			73	84	126	58
		1201	84	86	98	39	38
			111	115	114	34
			85	92	101	40
		1715	102	99	110	36	33
			88#	93	83	31
			95	86	88	31
		2450	104	97	90	29	32
			107	103	106	33
			99	96	97	33
		3500	98	92	103	35	34
			93	90	101	36
			106	108	101	32
		5000	95	97	90	31	34
			92	98	114	41
			85	87	75	29
Positive Control	3-Methylcholanthrene	5	82	75	368	150	155*
			75	70	364	162
			81	72	372	153

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	78	109	58	25	26
			79	105	58	24
			71	86	59	28
Test Chemical	Methyl carbamate	2048	70#	82	39	19
		2560	54	65	69	42	33
			65	76	46	24	33
		3200	64	98	36	19	25
			67	110	62	31	25
		4000	63	81	84	45
		5000	71	83	72	34	25
			72	85	36	17	25
Positive Control	3-Methylcholanthrene	5	59	54	234	132	132*
			56	53	232	137
			54	63	208	128

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination