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Study Information
Study Attribute Value
CEBS Accession Number: 002-02429-0011-0000-1
Chemical Name: Methyl carbamate
CASRN: 598-55-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 694447
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 82 104 44 18 19
    101 85 49 16
    94 90 59 21
    100 121 62 21
Test Chemical Methyl carbamate 
156         
 103 102 54 18 14
    101 91 31 10
  
313         
 100 84 56 19 17
    103 105 45 15
  
625         
 89# 94 21 8 13
    101 99 56 18
  
1250         
 104 102 50 16 15
    89 98 35 13
  
2500         
 96 91 67 23 20
    93 100 49 18
  
5000         
 95 89 38 13 13
    88 104 35 13
Positive Control Ethyl Methane Sulfonate 
500         
 33 17 877 877 730*
    40 24 893 741
    65 31 1113 572

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 76 97 46 20 16
    81 104 40 16
    72 91 41 19
    81 109 22 9
Test Chemical Methyl carbamate 
313         
 99 128 60 20 17
    89 79 52 20
    79 116 28 12
  
625         
 96 146 36 13 13
    107 145 47 15
    91 144 35 13
  
1250         
 106 145 36 11 10
    110 183 23 7
    102 163 32 10
  
2500         
 107 153 27 8 8
    114 164 28 8
    121r 169 33 9
  
5000         
 124r 141 27 7
    123r 157 37 10
    113 160 49 14
Positive Control Ethyl Methane Sulfonate 
500         
 65 62 681 349 492*
    36 25 614 577
    44 27 720 550

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 66 91 56 28 19
    79 119 48 20
    77 101 34 15
    86 89 34 13
Test Chemical Methyl carbamate 
313         
 80# 78 55 23 26
    69 79 65 31
    62 86 44 24
  
625         
 80 69 63 26 24
    57 65 36 21
    87 71 65 25
  
1250         
 82 80 41 17 20
    70 97 41 19
    73 72 52 24
  
2500         
 75 78 34 15 18
    100r 83 0 0
    89 76 54 20
  
5000         
 72# 79 31.5 15 20
    75 94 51 23
    71 74 48 23
Positive Control 3-Methylcholanthrene 
7         
 66 49 229 116 111*
    60 57 182 101
    79 60 273 115

Non-Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 109 119 52 16 17
    106# 95 51 16
    102# 85 45 15
    101 101 60 20
Test Chemical Methyl carbamate 
313         
 101 101 45 15 19
    86 81 47 18
    104 77 78 25
  
625         
 87 81 40 15 16
    84 85 42 17
    94 74 46 16
  
1250         
 87 93 39 15 16
    93# 90 48 17
    82 84 37 15
  
2500         
 104 117 47 15 16
    79 106 37 16
    86# 94 46.5 18
  
5000         
 82 91 52 21 18
    96 90 49 17
    83 83 37 15
Positive Control 3-Methylcholanthrene 
7         
 106 63 278 88 90*
    91 60 248 91
    87 57 236 90

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 112 90 86 26 22
    99# 118 78 26
    88 93 55 21
    80 98 35 15
Test Chemical Methyl carbamate 
156         
 98 101 57 19 23
    71 78 58 27
  
313         
 110 82 85 26
    127r 112 69 18
  
625         
 77# 102 48 21 23
    71 106 53 25
  
1250         
 100 98 95 32 27
    92 99 61 22
  
2500         
 99 103 58 20 20
    79# 96 49.5 21
  
5000         
 97 112 70 24 22
    104 94 63 20
Positive Control 3-Methylcholanthrene 
5         
 40 16 392 328 314*
    39 17 402 342
    47 23 385 273

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 75 92 32 14 15
    81 108 36 15
    82 99 28 11
    75 101 42 19
Test Chemical Methyl carbamate 
313         
 78 61 46 20 24*
    46 55 39 28
    62 77 44 24
  
625         
 41 44 31 26 28*
    78 89 43 18
    51 56 63 41
  
1250         
 79 79 69 29 22
    77 93 49 21
    68 71 30 15
  
2500         
 92 99 56 20 21
    81 118 43 18
    55 57 42 26
  
5000         
 64 88 39 20 20
    87 94 34 13
    70 81 57 27
Positive Control 3-Methylcholanthrene 
5         
 43 19 232 181 190*
    44 16 299 227
    51 19 248 164

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination