Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02432-0012-0000-6
Chemical Name:	Methyl dopa
CASRN:	555-30-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	921912
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	113	90	114	34	25
			108	86	61	19
			112	124	68	20
			115	121	95	28
Test Chemical	Methyl dopa	1.25	119	112	95	27	26
			118	166	80	23
			110	134	91	28
		2.5	109	147	109	33	31
			117	39	115	33
			103	85	87	28
		5	107	120	107	33	30
			102	124	78	26
			115	67	108	31
		7.5	115	97	82	24	27
			117	74	109	31
			114	110	91	27
Positive Control	Methyl Methane Sulfonate	5	58	26	484	277	202*
			58	28	228	130
			63	50	372	198

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	111	138	117	35	50
			80	83	151	63
			94	108	132	47
			64	71	105	55
Test Chemical	Methyl dopa	10	68	68	153	75	47
			110#	109	94	29
			111	79	120	36
		12	85	47	149	58	79*
			84	39	229	91
			96	13	249	87
		13	100	30	323	108	92*
			128r	67	162	42
			115	58	259	75
		14	110	69	149	45	96*
			80	5	366	153
			103	43	275	89
		15

		16


Positive Control	Methyl Methane Sulfonate	5	71#	43	485	227	224*
			58	47	396	227
			69#	62	456	219

Nonactivation Trial: 3 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	107	82	77	24	31
			110	99	93	28
			107#	106	115	36
			112	113	115	34
Test Chemical	Methyl dopa	10	109	97	88	27	24
			114	131	97	28
			115	87	55	16
		12	111	108	105	32	26
			111	116	97	29
			114	135	54	16
		14	124r	121	62	17	25
			112	82	81	24
			109	98	83	25
		15	115	128	107	31	30
			115	91	115	33
			116	80	88	25
		16	113	93	102	30	29
			118	94	93	26
			112	98	98	29
		20	110	105	106	32	30
			117	100	95	27
			109	104	101	31
Positive Control	Methyl Methane Sulfonate	5	107	69	481	151	135*
			105	76	288	91
			92	40	445	162

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	115	97	60	17	18
			89	104	52	20
			118	123	51	14
			120	76	72	20
Test Chemical	Methyl dopa	2.5	114	122	51	15	20
			109	38	87	27
			110	124	62	19
		5	121r	129	42	12
			115	83	68	20
			128r	159	47	12
		7.5	122r	106	47	13	16
			101	107	47	16
			92	81	44	16
		10	120	127	57	16	16
			105	116	58	18
			102	85	43	14
		15


Positive Control	Methyl Methane Sulfonate	5	113	72	272	80	126*
			70	52	376	180
			105	69	370	117

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	84	93	241	95	97
			74	114	183	82
			87	111	271	104
			63	82	200	107
Test Chemical	Methyl dopa	5	95	80	145	51	59
			96	61	180	63
			88	70	165	62
		10	100	76	132	44	47
			72	43	108	50
			92	80	128	47
		15	88	87	166	63	70
			98	87	234	79
			99	74	202	68
		20	117	90	302	86	70
			107	75	169	53
			91	79	198	72
		30	95	94	218	77	66
			103	79	208	68
			101	28	158	52
		40	65	10	446	229	261*
			71	17	554	262
			58	8	512	293
Positive Control	Methyl Methane Sulfonate	5	53	44	815	514	515*
			61	51	961	524
			62	47	933	506

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	80	84	155	65	54
			110	128	134	41
			85	126	96	38
			68	62	148	73
Test Chemical	Methyl dopa	20	69	75	121	59	52
			84	118	122	48
			79	93	116	49
		40	99	110	109	37	47
			92	83	144	52
			80	62	123	51
		50	90	120	118	44	45
			105	108	159	50
			83	110	98	40
		75	103	123	158	51	47
			117	121	135	39
			64	61	97	51
		100	111	98	124	37	41
			111	92	126	38
			68	67	97	47
		150	104	141	138	44	54
			72	86	154	71
			92	78	126	46
Positive Control	3-Methylcholanthrene	2.5	92	87	609	221	245*
			54	52	481	295
			104	78	683	220

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	102	92	125	41	48
			88	93	122	46
			98	99	133	45
			90#	116	162	60
Test Chemical	Methyl dopa	100	97	29	118	41	58
			128r	62	137	36
			64	14	144	75
		200	109	21	125	38	51
			103	21	162	53
			69	10	125	61
		300	123r	45	276	75	96*
			89	25	302	113
			86	12	202	78
		400					149*
			66	9	327	165
			38	3	151	132
		500


Positive Control	3-Methylcholanthrene	2.5	54	35	605	373	324*
			57	26	497	291
			57	40	522	308

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	107	110	198	61	64
			76	69	150	66
			87	98	169	65
			87	123	162	62
Test Chemical	Methyl dopa	100	83	84	139	56	48
			102	113	157	52
			100	102	110	37
		200	103	88	99	32	36
			95	74	95	33
			112	88	144	43
		300	69	48	230	111	72
			97	89	127	44
			88	82	159	60
		400					154*
			111	11	512	154
			106	17	487	153
		500


Positive Control	3-Methylcholanthrene	2.5	49	26	638	431	459*
			45#	19	718	536
			51#	18	625	410

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination