Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02434-0014-0000-0
Chemical Name:	Methyl ethyl ketone peroxide
CASRN:	1338-23-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	091264
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	78#	113	71	31	32
			65	103	69	35
			50	57	44	29
			104	128	100	32
Test Chemical	Methyl ethyl ketone peroxide	2	39	38	111	94	87*
			40	44	123	103
			72	60	136	63
		3	35	40	88	83	78*
			29	25	88	99
			57	51	88	52
		4	61	60	385	211	190*
			46	32	273	200
			30#	39	143	158
		6	70	75	319	152	182*
			66#	38	390	197
			39	55	229	198
		8	59	19	396	223	223*
			61	34	408	222	223*
		10	65	23	495	254	222*
			84	29	481	191	222*
Positive Control	Methyl Methane Sulfonate	5	52	59	448	284	266*
			57	41	505	295
			63	66	417	219

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	85	88	132	52	43
			95	98	127	45
			130r	103	130	33
			118	114	118	33
Test Chemical	Methyl ethyl ketone peroxide	0.625	84	88	84	33	32
			85	81	81	32
			113#	98	106	31
		1.25	91	100	98	36	36
			107	92	106	33
			91	99	108	39
		2.5	97#	86	108	37	69*
			91	58	266	97
			85	65	182	71
		5	73	35	481	221	170*
			75	36	227	101
			75	41	424	188
		7.5	79#	31	544	230	267*
			86	23	776	300
			93	27	761	272
		10	74	23	616	279	306*
			75	16	740	327
			74	12	696	313
Positive Control	Methyl Methane Sulfonate	5	84	69	601	238	256*
			81#	63	604	250
			76	62	640	279

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination