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Study Information
Study Attribute Value
CEBS Accession Number: 002-02442-0013-0000-8
Chemical Name: Methyl methacrylate
CASRN: 80-62-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 438144
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 113 110 120 35 36
    100 74 120 40
    101 111 88 29
    108 105 128 39
Test Chemical Methyl methacrylate 
 0.25      
 100 69 77 26 26
    113 71 83 25
    91 83 74 27
  
 0.5       
 120 56 117 33 25
    111 62 72 22
    106 53 66 21
  
 0.75      
 109 36 155 47 40
    93 34 114 41
    93 44 88 31
  
1         
 96 32 137 48 44
    117 36 137 39
    92 40 125 46
  
1.5       
 90 20 206 77 80*
    80 16 204 85
    103 16 240 78
  
2         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 58 28 949 549 446*
    58 35 713 406
    64 53 740 384

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 71 84 96 45 39
    70 101 104 50
    85 115 60 24
Test Chemical Methyl methacrylate 
 0.125     
 71 102 59 28 43
    71 52 119 56
    74 35 97 44
  
 0.25      
 74 68 65 29 38
    67 65 82 41
    73 85 96 44
  
 0.5       
 65 52 162 83 68*
    58 41 96 55
    49 33 99 67
  
 0.75      
 44 7 366 276 203*
    39 13 226 194
    61 22 252 139
  
1         
 54 6 482 299 281*
    41 5 381 307
    48 13 339 237
  
1.5       
   
   
Positive Control Methyl Methane Sulfonate 
5         
 53 52 617 388 337*
    62 45 691 373
    63 39 473 249

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 60 102 207 116 102
    71 107 168 79
    61 103 167 92
    58 88 207 120
Test Chemical Methyl methacrylate 
 0.125     
 79 94 365 154 145
    66 98 265 134
    60 88 267 148
  
 0.25      
 49 70 305 210 227*
    51 63 374 247
    54 82 362 225
  
 0.5       
 64 75 480 251 273*
    54 63 531 326
    63# 61 460 242
  
1         
 55 51 559 342 343*
    59 36 557 313
    52 43 588 373
  
1.5       
 54 20 663 408 376*
    50 28 552 372
    54 28 567 348
  
2         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 33 29 677 680 688*
    37 7 694 628
    27 6 608 755

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination