Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02442-0013-0000-8
Chemical Name:	Methyl methacrylate
CASRN:	80-62-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	438144
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	113	110	120	35	36
			100	74	120	40
			101	111	88	29
			108	105	128	39
Test Chemical	Methyl methacrylate	0.25	100	69	77	26	26
			113	71	83	25
			91	83	74	27
		0.5	120	56	117	33	25
			111	62	72	22
			106	53	66	21
		0.75	109	36	155	47	40
			93	34	114	41
			93	44	88	31
		1	96	32	137	48	44
			117	36	137	39
			92	40	125	46
		1.5	90	20	206	77	80*
			80	16	204	85
			103	16	240	78
		2


Positive Control	Methyl Methane Sulfonate	5	58	28	949	549	446*
			58	35	713	406
			64	53	740	384

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	71	84	96	45	39
			70	101	104	50
			85	115	60	24
Test Chemical	Methyl methacrylate	0.125	71	102	59	28	43
			71	52	119	56
			74	35	97	44
		0.25	74	68	65	29	38
			67	65	82	41
			73	85	96	44
		0.5	65	52	162	83	68*
			58	41	96	55
			49	33	99	67
		0.75	44	7	366	276	203*
			39	13	226	194
			61	22	252	139
		1	54	6	482	299	281*
			41	5	381	307
			48	13	339	237
		1.5


Positive Control	Methyl Methane Sulfonate	5	53	52	617	388	337*
			62	45	691	373
			63	39	473	249

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	60	102	207	116	102
			71	107	168	79
			61	103	167	92
			58	88	207	120
Test Chemical	Methyl methacrylate	0.125	79	94	365	154	145
			66	98	265	134
			60	88	267	148
		0.25	49	70	305	210	227*
			51	63	374	247
			54	82	362	225
		0.5	64	75	480	251	273*
			54	63	531	326
			63#	61	460	242
		1	55	51	559	342	343*
			59	36	557	313
			52	43	588	373
		1.5	54	20	663	408	376*
			50	28	552	372
			54	28	567	348
		2


Positive Control	3-Methylcholanthrene	2.5	33	29	677	680	688*
			37	7	694	628
			27	6	608	755

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination