Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02460-0001-0000-5
Chemical Name:	Methylazoxymethanol acetate
CASRN:	592-62-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	413574
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	110	144	72	77
			69	104	170	82
			50#	87	125	83
			78	99	164	70
Test Chemical	Methylazoxymethanol acetate	0.0025	56#	83	173	103	112*
			66	86	252	128
			62	90	194	105
		0.005	52#	68	255	164	152*
			58	69	277	160
			49	70	193	131
		0.01	39#	54	290	249	272*
			41	56	374	303
			51	52	403	263
		0.02	34	35	328	325	359*
			31#	33	321	351
			30	37	364	400
		0.03	17	18	293	580	572*
			21	20	355	563	572*
		0.04	13	14	246	623	614*
			14	13	260	605	614*
Positive Control	Ethyl Methane Sulfonate	250	5r	2	111	716
			1r	0	107	3057
			2r	0	87	1933

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	96	43	19	19
			110#	109	60	18
			129r	163	51	13
			88	95	53	20
Test Chemical	Methylazoxymethanol acetate	0.002	63	61	99	53	38*
			76#	78	71	31
			74	71	69	31
		0.005	55	55	75	45	57*
			50	53	104	69	57*
		0.01	71	58	202	95	112*
			61	42	234	129	112*
		0.02	37	19	265	241	244*
			42	25	307	247	244*
		0.03	32	19	273	281	317*
			16	7	152	327
			15	7	151	343
		0.04	13	5	211	555
			10r	4	215	729
Positive Control	Ethyl Methane Sulfonate	250	81	65	767	316	340*
			91#	61	984	361
			77	57	793	342

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	113	90	29	35
			88	93	137	52
			81	105	57	23
			81	88	86	35
Test Chemical	Methylazoxymethanol acetate	0.313	88	90	82	31
		0.625	71	71	156	73	66*
			63	68	112	60	66*
		1.25	67#	63	229.5	114	116*
			72	69	252	117	116*
		2.5	65	50	317	164	203*
			60	39	440	243	203*
		5	40	27	308	255	239*
			49	24	325	223	239*
Positive Control	3-Methylcholanthrene	5	44	12	296	227	268*
Positive Control			43	11	401	308	268*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	93	172	69	64
			84	120	141	56
			80	94	147	62
			94	93	191	67
Test Chemical	Methylazoxymethanol acetate	0.156	64	67	223	117	103*
			76	74	211	93
			78	74	233	100
		0.313	78#	68	268	115	121*
			57	73	231	136
			61	57	208	113
		0.625	58	64	219	126	131*
			66	66	258	131
			67	65	272	135
		1.25	58	50	337	194	154*
			58	48	261	149
			77	45	274	119
		2.5	43	33	340	265	256*
			32	22	267	280
			39	27	261	224
		5	15	9	192	417	423*
			19	11	207	373
			17	10	242	479
Positive Control	3-Methylcholanthrene	2.5	38	14	355	309	282*
			37	14	316	287
			44	13	331	249

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination