Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02460-0002-0000-6
Chemical Name:	Methylazoxymethanol acetate
CASRN:	592-62-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	665199
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	59	96	100	56	59
			61	95	105	57
			64	114	139	73
			61	95	89	49
Test Chemical	Methylazoxymethanol acetate	0.003	49	84	188	129	127*
			46	65	170	125	127*
		0.005	52	64	187	121	146*
			54	65	278	172	146*
		0.007	36	39	222	208	228*
			26	29	195	248	228*
		0.01	15	16	224	492	438*
			17	22	196	384	438*
		0.014	17	16	179	348	328*
			17	15	160	308	328*
		0.02	8r	6	145	630
			7r	6	118	562
Positive Control	Ethyl Methane Sulfonate	500	36	36	534	499	442*
			39	45	499	423
			37	41	446	405

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	61	100	134	73	62
			64	100	114	60
			59	100	98	56
			65	99	121	62
Test Chemical	Methylazoxymethanol acetate	0.001	42	61	46	37	39
			42	71	51	41	39
		0.002	41	68	62	50	48
			41	64	57	47	48
		0.004	31	43	76	82	76
			34	43	71	70	76
		0.008	24	25	104	146	114*
			33	37	80	81	114*
		0.01	23	24	104	149	125*
			29	30	87	101	125*
		0.013	18	17	110	204	215*
			17	16	117	227	215*
		0.016	12	10	84	227	192*
			19	17	89	158	192*
		0.02	6r	7	53	286
			11	9	95	284
Positive Control	Ethyl Methane Sulfonate	500	39	37	663	564	619*
			40	41	716	602
			34	36	697	690

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	61	97	119	65	71
			56	93	115	69
			58	102	110	63
			60	108	156	87
Test Chemical	Methylazoxymethanol acetate	0.002	39	55	246	209	202*
			41	51	237	194	202*
		0.003	39	44	240	206	192*
			49	59	263	179	192*
		0.005	26	22	219	279	292*
			21	20	188	306	292*
		0.007	13	11	149	387	451*
			13	10	206	515	451*
		0.01	4r	3	102	816
			4r	3	102	816
		0.014	2r	1	91	1400
			2r	1	97	1940
		0.02	1r	1	81	2700
			1r	1	59	2360
Positive Control	3-Methylcholanthrene	6	21	8	313	505	488*
			17	5	221	438
			17	6	266	522

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	106	101	196	62	57
			107#	102	202.5	63
			108	104	161	50
			103	94	166	54
Test Chemical	Methylazoxymethanol acetate	0.0013	75	62	581	258	240*
			89	73	594	223	240*
		0.0017	77	58	757	328	298*
			85	70	678	267	298*
		0.0021	83	62	771	312	324*
			77	60	776	337	324*
		0.0026	71	49	821	384	362*
			79	55	811	341	362*
		0.0033	67	42	940	471	424*
			67#	41	753	376	424*
		0.0041	71	47	832	391	417*
			61	40	810	443	417*
		0.0051	57	35	811	473	439*
			60#	40	730	406	439*
		0.0064	46	28	777	567	549*
			42	27	662	532	549*
		0.008	29	16	644	740	647*
			32#	18	536	554	647*
Positive Control	3-Methylcholanthrene	5	83	60	815	329	363*
			73	51	885	403
			78#	56	830	357

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination