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Study Information
Study Attribute Value
CEBS Accession Number: 002-02465-0002-0000-1
Chemical Name: Methylene chloride
CASRN: 75-09-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 676332_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #:1   Activation: No Activation    Date: 06/14/1988    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Methylene chloride 
1600         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
3000         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5000         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 23 0.115 11.0 10 0.050 5.0 13 0.065 6.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 14 0.280 26.0 9 0.180 18.0 5 0.100 10.0 0 0.000 0.0
Trend: 0.594 0.000 0.594  
Probability: 0.276 0.000 0.276  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 06/14/1988    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Test Chemical: Methylene chloride 
1600         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
3000         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
5000         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 24 0.120 10.5 2 0.010 1.0 22 0.110 9.5 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 17 0.340 30.0 5 0.100 10.0 12 0.240 24.0 0 0.000 0.0
Trend: -0.443 -1.351 0.269  
Probability: 0.671 0.912 0.394