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Study Information
Study Attribute Value
CEBS Accession Number: 002-02466-0001-0000-1
Chemical Name: Methylenedianiline
CASRN: 101-77-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 321885_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 02/11/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 304 0.291 6.08 26.0 0.000
Test Chemical Methylenedianiline 
5         
 50 1050 376 0.358 7.52 26.0 23.213 
16.7       
 50 1048 483 0.461 9.66 26.0 58.578 
50         
 50 1046 357 0.341 7.14 26.0 17.434 
166.7       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1047 508 0.485 10.16 26.0 66.946
Mitomycin-C 
 0.01      
 5 105 211 2.010 42.20 26.0 591.435
Trend: 3.170        
Probability: 0.001        

Trial #: 2    Activation: No Activation    Date: 02/25/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 524 179 0.342 7.16 25.5 0.000
Test Chemical Methylenedianiline 
10         
 25 518 230 0.444 9.20 25.5 29.980 
15         
 25 522 267 0.511 10.68 34.2 49.734 
20         
 25 514 293 0.570 11.72 34.2 66.872 
50.1       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 25 522 203 0.389 8.12 25.5 13.842
Mitomycin-C 
 0.01      
 5 104 177 1.702 35.40 25.5 398.217
Trend: 5.669        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/11/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 311 0.298 6.22 26.2 0.000
Test Chemical Methylenedianiline 
50         
 50 1049 328 0.313 6.56 26.2 4.863 
166.7       
 50 1045 374 0.358 7.48 26.2 20.027 
500         
 45 934 413 0.442 9.18 26.2 48.295 
1700         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1048 575 0.549 11.50 26.2 84.005
Cyclophosphamide 
2         
 5 103 194 1.883 38.80 26.2 531.667
Trend: 5.580        
Probability: 0.000