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Study Information
Study Attribute Value
CEBS Accession Number: 002-02466-0002-0000-2
Chemical Name: Methylenedianiline
CASRN: 101-77-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 321885_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #:1   Activation: No Activation    Date: 03/25/1987    Harvest Time: 20.2 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Test Chemical: Methylenedianiline 
75.4       
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
112.5       
 200 4 0.020 2.0 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0 
150         
 200 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 24 0.120 10.5 10 0.050 5.0 14 0.070 6.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 10 0.400 36.0 4 0.160 16.0 6 0.240 24.0 0 0.000 0.0
Trend: -0.063 0.275 -0.272  
Probability: 0.525 0.392 0.607  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/08/1987    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 2 0.010 1.0 1 0.005 0.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Test Chemical: Methylenedianiline 
101         
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 
252         
 200 6 0.030 3.0 5 0.025 2.5 1 0.005 0.5 0 0.000 0.0 
502.5       
 200 9 0.045 4.5 1 0.005 0.5 8 0.040 4.0 0 0.000 0.0 
750         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 29 0.145 12.0 4 0.020 2.0 25 0.125 10.5 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 13 0.520 40.0 1 0.040 4.0 12 0.480 36.0 0 0.000 0.0
Trend: 1.933 0.520 2.066  
Probability: 0.027 0.301 0.019