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Study Information
Study Attribute Value
CEBS Accession Number: 002-02469-0003-0000-6
Chemical Name: Methylphenidate hydrochloride
CASRN: 298-59-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 864605_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 01/05/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Water 
0         
 50 1034 388 0.375 7.76 26.0 0.000
Test Chemical Methylphenidate hydrochloride 
702         
 50 1013 464 0.458 9.28 36.6 22.067 
800         
 50 1010 505 0.500 10.10 36.6 33.247 
900         
 50 1016 575 0.566 11.50 36.6 50.821 
1000         
 0 0 0 0.000 0.00 36.6 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1023 644 0.630 12.88 26.0 67.764
Mitomycin-C 
 0.01      
 5 105 211 2.010 42.20 26.0 435.528
Trend: 6.368        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 12/14/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Water 
0         
 50 1031 391 0.379 7.82 25.7 0.000
Test Chemical Methylphenidate hydrochloride 
1400         
 50 1035 448 0.433 8.96 25.7 14.135 
1600         
 50 1041 470 0.451 9.40 25.7 19.050 
2000         
 50 1029 474 0.461 9.48 25.7 21.463
Positive Control Cyclophosphamide 
 0.3       
 50 1025 474 0.462 9.48 25.7 21.937
Cyclophosphamide 
2         
 5 104 119 1.144 23.80 25.7 201.714
Trend: 2.820        
Probability: 0.002        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 12/28/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1005 412 0.410 8.24 25.3 -19.973
Water 
0         
 50 1019 522 0.512 10.44 25.3 0.000
Test Chemical Methylphenidate hydrochloride 
1500         
 50 1020 538 0.527 10.76 25.3 2.964 
1750         
 50 1015 583 0.574 11.66 25.3 12.126 
2000         
 50 1020 594 0.582 11.88 25.3 13.682 
2500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1008 678 0.673 13.56 25.3 31.302
Cyclophosphamide 
2         
 5 104 250 2.404 50.00 25.3 369.257
Trend: 2.484        
Probability: 0.006