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Study Information
Study Attribute Value
CEBS Accession Number: 002-02469-0003-0000-6
Chemical Name: Methylphenidate hydrochloride
CASRN: 298-59-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 864605_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 01/05/1983    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Water
0         
50 1034 388 0.375 7.76 26.0 0.000
Test Chemical Methylphenidate hydrochloride
702         
50 1013 464 0.458 9.28 36.6 22.067
800         
50 1010 505 0.500 10.10 36.6 33.247
900         
50 1016 575 0.566 11.50 36.6 50.821
1000         
0 0 0 0.000 0.00 36.6 N/A
Positive Control Mitomycin-C
 0.001     
50 1023 644 0.630 12.88 26.0 67.764
Mitomycin-C
 0.01      
5 105 211 2.010 42.20 26.0 435.528
Trend: 6.368        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 12/14/1982    Trial Call: Weakly Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Water
0         
50 1031 391 0.379 7.82 25.7 0.000
Test Chemical Methylphenidate hydrochloride
1400         
50 1035 448 0.433 8.96 25.7 14.135
1600         
50 1041 470 0.451 9.40 25.7 19.050
2000         
50 1029 474 0.461 9.48 25.7 21.463
Positive Control Cyclophosphamide
 0.3       
50 1025 474 0.462 9.48 25.7 21.937
Cyclophosphamide
2         
5 104 119 1.144 23.80 25.7 201.714
Trend: 2.820        
Probability: 0.002        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 12/28/1982    Trial Call: Negative   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified)
0         
50 1005 412 0.410 8.24 25.3 -19.973
Water
0         
50 1019 522 0.512 10.44 25.3 0.000
Test Chemical Methylphenidate hydrochloride
1500         
50 1020 538 0.527 10.76 25.3 2.964
1750         
50 1015 583 0.574 11.66 25.3 12.126
2000         
50 1020 594 0.582 11.88 25.3 13.682
2500         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
 0.3       
50 1008 678 0.673 13.56 25.3 31.302
Cyclophosphamide
2         
5 104 250 2.404 50.00 25.3 369.257
Trend: 2.484        
Probability: 0.006