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Study Information
Study Attribute Value
CEBS Accession Number: 002-02476-0010-0000-2
Chemical Name: Michler's ketone
CASRN: 90-94-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G29620
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 131 46 18 26
    71 87 68 32
    63 68 62 33
    90 115 56 21
Test Chemical Michler's ketone 
6         
 83 81 47 19 22
    78 87 60 26
  
7         
 74 69 57 26 24
    92 64 64 23
  
8         
 77 57 88 38 35
    81 73 79 33
  
9         
 80 70 81 34 33
    78 55 73 31
  
10         
 63 51 111 59 55*
    74 55 113 51
  
12         
 68 39 140 69 67*
    82# 45 163.5 66
Positive Control Ethyl Methane Sulfonate 
500         
 41 31 675 547 547*
    34 28 618 603
    44 39 647 490

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 96 44 17 16
    98 121 37 13
    74 80 49 22
    83 104 36 14
Test Chemical Michler's ketone 
1.25      
 109 64 79 24 26*
    106 87 80 25
    82 51 67 27
  
2.5       
 76 59 50 22 18
    87 57 41 16
    85 81 45 18
  
5         
 91 76 63 23 21
    100 79 52 17
    93 61 62 22
  
7.5       
 68 49 38 19 20
    83 66 52 21
    101 53 60 20
  
10         
 74 46 50 23 21
    82 58 53 22
    92 85 54 20
  
12         
 22
    72 37 47 22
    76 59 52 23
  
15         
   
   
Positive Control Ethyl Methane Sulfonate 
500         
 52 36 743 472 558*
    41 31 606 497
    36 22 761 705

Nonactivation Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 111 52 20 17
    96 102 61 21
    89# 92 42 16
    78 95 24 10
Test Chemical Michler's ketone 
2         
 90 48 48 18
  
4         
 45 31 51 38 29*
    82 36 63 26
    80 37 53 22
  
8         
 74 49 48 22 25
    46 24 34 25
    40 22 36 30
  
10         
 51 26 49 32 37*
    50 27 38 25
    49 22 80 55
  
12         
 58 34 35 20 22
    55 24 56 34
    70 25 24 11
  
14         
 65 26 63 32 35*
    51 19 61 40
    57 27 57 33
Positive Control Ethyl Methane Sulfonate 
500         
 8r 1 261 1088 1020*
    12 3 362 1020

Nonactivation Trial: 4 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 97 35 16 21
    61 89 53 29
    88 119 51 19
    76 94 43 19
Test Chemical Michler's ketone 
4         
 56# 44 33 20 19
    49 45 33 23
    52 34 24 15
  
8         
 53 40 22 14 16
    48 36 15 11
    35 33 26 25
  
10         
 50# 29 38 25 24
    62 39 47 25
    58 36 36 21
  
12         
 46 35 26 19 21
    51 33 36 24
  
14         
 61 33 21 12 19
    48 30 31 22
    64 39 43 22
  
16         
 53 43 34 21 23
    64 39 47 25
Positive Control Ethyl Methane Sulfonate 
500         
 16 9 678 1413 1226*
    17 10 681 1310
    30# 25 850.5 956

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 106 91 29 30
    84# 100 85.5 34
    79 80 81 34
    78 114 56 24
Test Chemical Michler's ketone 
 0.5       
 92 107 44 16 23
    92 76 84 30
  
1         
 98# 26 132 45 47
    77 65 111 48
  
2         
 94 48 113 40 41
    88 47 108 41
  
4         
 77 46 159 69 59*
    98 51 147 50
  
8         
 77 15 294 127 120*
    88 28 298 112
  
16         
   
Positive Control 3-Methylcholanthrene 
5         
 43 18 380 293 272*
    60 29 448 250

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 114 110 98 29 34
    101 109 112 37
    71 66 87 41
    113 116 100 30
Test Chemical Michler's ketone 
4         
 104 81 246 79 82*
    70 61 179 85
  
6         
 71 52 306 145 126*
    97 58 313 108
  
8         
 83 35 377 152 168*
    73 22 406 185
  
10         
 68 15 495 243 243*
    58 19 428 244
  
12         
 49 9 534 365 360*
    49 9 523 355
  
15         
 38 4 422 369 375*
    36 4 406 381
Positive Control 3-Methylcholanthrene 
5         
 45 27 496 369 328*
    54 24 540 334
    66 37 558 280

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination