Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02476-0011-0000-3
Chemical Name:	Michler's ketone
CASRN:	90-94-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G71544
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	51	89	69	45	37
			61	94	61	33
			70	117	66	31
			48r	64	54	37
Test Chemical	Michler's ketone	8.3	75	158	76	34	31
			48	96	42	29	31
		9.8	52	37	69	44	40
			48	40	51	35	40
		11.5	50	34	56	38	39
			50	38	60	40	39
		13.5	35	20	81	76	68*
			37	20	65	59	68*
		15.9	20	10	59	98	80*
			33	19	61	62	80*
		18.7
		22

Positive Control	Ethyl Methane Sulfonate	500	37	38	750	679	600*
			33	33	698	716
			68	87	820	404

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	105	55	27	22
			70	96	37	18
			72	103	44	20
			73	96	53	24
Test Chemical	Michler's ketone	9.6	65	72	83	42	32
			72	82	39	18
			64	68	67	35
		10.6	63	73	54	28	28
			61	73	56	31
			62	68	46	25
		11.8	67	76	80	40	33
			65	72	80	41
			67	75	37	18
		13.1	70	72	62	29	35*
			62	68	56	30
			57	56	77	45
		14.6	49	51	57	39	56*
			27	14	63	78
			41	29	62	50
		16.2
		18


Positive Control	Ethyl Methane Sulfonate	500	66	99	487	247	277*
			58	83	466	268
			54	83	513	316

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	110	282	108	117
			83	106	273	110
			84#	91	305	121
			82	94	321	131
Test Chemical	Michler's ketone	8.2	108	93	468	145	138
			111	75	438	132	138
		10.2	64	43	487	255	215*
			94	55	493	175	215*
		12.8	73	28	420	193	218*
			89	28	644	242	218*
		16	73	16	659	303	308*
			68	14	639	313	308*
		20	41	8	670	545	539*
			40	6	645	533	539*
		25	24	4	548	766	666*
			30	4	512	566	666*
Positive Control	3-Methylcholanthrene	5	64	63	656	342	384*
			60	55	720	400
			55	60	671	410

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79#	109	166	70	71
			82	100	183	74
			79	107	142	60
			66#	84	157	79
Test Chemical	Michler's ketone	8.2	60	83	223	125	130*
			73	72	294	135	130*
		10.2	63	52	275	146	161*
			65	56	341	175	161*
		12.8	57	28	383	225	220*
			58	16	376	214	220*
		16	51	16	503	330	313*
			51	14	449	295	313*
		20	41	7	434	357	314*
			52#	11	426	271	314*
		25	21	3	457	714	653*
			23	3	405	591	653*
Positive Control	3-Methylcholanthrene	5	50	44	536	359	347*
			57	49	587	344
			59	51	599	337

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	91	108	279	102	105
			83	97	248	99
			92	100	264	96
			86	95	320	124
Test Chemical	Michler's ketone	6.6	85	83	383	150	146
			97	71	414	143	146
		8.2	86	82	435	168	173*
			79	74	417	177	173*
		10.2	82	64	449	182
		12.8	75	51	506	224	244*
			63	43	502	265	244*
		16	61	25	532	289	289*
			58	23	499	288	289*
		20	48	13	673	471	405*
			55	15	563	339	405*
		25

Positive Control	3-Methylcholanthrene	5	52	39	579	369	374*
			52	36	591	380
			53	38	589	373

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination