Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02485-0010-0000-2
Chemical Name:	Monochloroacetic acid
CASRN:	79-11-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	486223
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	79	104	106	45	40
			80	107	68	28
			82	96	91	37
			93	93	136	49
Test Chemical	Monochloroacetic acid	50	84	85	123	49	46
			94	81	121	43	46
		100	112	75	135	40	38
			83	77	88	35	38
		200	89	75	127	47	60
			84	74	181	72	60
		400	55	15	149	90	122*
			52	15	239	153	122*
		800	80	28	218	90	114*
			67	19	275	137	114*
Positive Control	Ethyl Methane Sulfonate	250	66	58	688	347	325*
Positive Control			76	62	688	302	325*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	71	93	84	40	46
			71	88	97	46
			56	113	80	48
			61	106	93	51
Test Chemical	Monochloroacetic acid	31.25	57	79	112	66	55
			66	100	88	45	55
		62.5	61	98	97	53	56
			49	82	86	59	56
		125	44	66	85	64	51
			100	91	116	39	51
		250	77	73	141	61	59
			64	59	108	57	59
		500

Positive Control	Ethyl Methane Sulfonate	250	70	74	683	327	331*
Positive Control			50	56	499	336	331*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	90	125	200	74	69
			81	98	185	77
			93	91	155	56
			76	87	157	69
Test Chemical	Monochloroacetic acid	100	77	80	166	72	75
			75	82	170	76
			77	77	178	77
		200	73	62	220	101	93
			73	56	184	84
			76	51	214	94
		400					184*
			50	7	299	198
			62	8	315	171
		600


Positive Control	Ethyl Methane Sulfonate	250	66	81	609	306	288*
Positive Control			84	64	685	271	288*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination