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Study Information
Study Attribute Value
CEBS Accession Number: 002-02492-0010-0000-0
Chemical Name: Monuron
CASRN: 150-68-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 455773
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 94 109 109 39 32
    107 96 81 25
    93 96 97 35
    95 99 81 29
Test Chemical Monuron 
50         
 92 87 95 35 31
    86 88 73 28
  
100         
 95 92 69 24 32
    92 82 110 40
  
200         
 99 79 102 34 41
    90 83 130 48
  
400         
 84 75 106 42 40
    101 59 114 38
  
800         
 120 29 126 35 43
    100 15 152 50
  
1600         
   
Positive Control Methyl Methane Sulfonate 
15         
 66 33 288 145 152*
    51 22 241 159
Methyl Methane Sulfonate 
250         
 85 72 369 146 157*
    70 74 351 168

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 54 94 107 66 60
    48r 74 111 78
    64 107 94 49
    57 99 112 65
Test Chemical Monuron 
350         
 70 82 125 60 68
    87 107 197 75
  
550         
 78 80 149 64 62
    76 92 139 61
  
750         
 71 67 101 47 54
    61 52 111 61
  
950         
 80 22 134 56 44
    83 37 79 32
Positive Control Methyl Methane Sulfonate 
15         
 38 34 391 343 360*
    37 27 421 378
Methyl Methane Sulfonate 
250         
 71 108 576 272 242*
    71 96 448 211

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 81 96 119 49 43
    79 100 112 47
    84 107 103 41
    104 96 110 35
Test Chemical Monuron 
150         
 84 74 114 45 56
    62 69 123 66
  
350         
 65 51 93 48 49
    65 55 99 51
  
550         
 76 76 126 55 55
    79 78 130 55
  
750         
 71 56 134 63 65
    79 58 160 68
  
950         
 87 61 173 66 69*
    68 50 146 72
Positive Control 3-Methylcholanthrene 
2.5       
 50 34 615 409 375*
    55 47 566 341

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 94 92 85 30 30
    83 102 91 37
    81 99 64 26
    79 107 59 25
Test Chemical Monuron 
150         
 69 72 117 56 48*
    81 103 96 40
  
350         
 59 75 77 44 46
    62 83 88 47
  
550         
 72 79 114 53 59*
    58 77 112 65
  
750         
 71 42 114 54 55*
    63 33 106 57
  
950         
 91 34 115 42 45
    79 20 115 48
Positive Control 3-Methylcholanthrene 
2.5       
 62 51 520 280 257*
    65 52 456 234

Induced S9 Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 105 139 64 66
    79 92 160 68
    75 107 166 74
    74 96 130 58
Test Chemical Monuron 
300         
 95 136 173 61 58
    73 88 123 56
  
500         
 70 84 158 75 72
    72 74 148 69
  
700         
 78 40 178 76 74
    74 42 159 72
  
900         
 58 16 117 68 75
    72 12 176 82
  
1100         
 60 9 128 71 73
    58 10 131 75
Positive Control 3-Methylcholanthrene 
2.5       
 33 22 427 438 406*
    41 27 454 374

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination