Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02492-0010-0000-0
Chemical Name:	Monuron
CASRN:	150-68-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	455773
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	109	109	39	32
			107	96	81	25
			93	96	97	35
			95	99	81	29
Test Chemical	Monuron	50	92	87	95	35	31
			86	88	73	28	31
		100	95	92	69	24	32
			92	82	110	40	32
		200	99	79	102	34	41
			90	83	130	48	41
		400	84	75	106	42	40
			101	59	114	38	40
		800	120	29	126	35	43
			100	15	152	50	43
		1600

Positive Control	Methyl Methane Sulfonate	15	66	33	288	145	152*
			51	22	241	159	152*
	Methyl Methane Sulfonate	250	85	72	369	146	157*
			70	74	351	168	157*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	54	94	107	66	60
			48r	74	111	78
			64	107	94	49
			57	99	112	65
Test Chemical	Monuron	350	70	82	125	60	68
			87	107	197	75	68
		550	78	80	149	64	62
			76	92	139	61	62
		750	71	67	101	47	54
			61	52	111	61	54
		950	80	22	134	56	44
			83	37	79	32	44
Positive Control	Methyl Methane Sulfonate	15	38	34	391	343	360*
			37	27	421	378	360*
	Methyl Methane Sulfonate	250	71	108	576	272	242*
			71	96	448	211	242*

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	96	119	49	43
			79	100	112	47
			84	107	103	41
			104	96	110	35
Test Chemical	Monuron	150	84	74	114	45	56
			62	69	123	66	56
		350	65	51	93	48	49
			65	55	99	51	49
		550	76	76	126	55	55
			79	78	130	55	55
		750	71	56	134	63	65
			79	58	160	68	65
		950	87	61	173	66	69*
			68	50	146	72	69*
Positive Control	3-Methylcholanthrene	2.5	50	34	615	409	375*
Positive Control			55	47	566	341	375*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	92	85	30	30
			83	102	91	37
			81	99	64	26
			79	107	59	25
Test Chemical	Monuron	150	69	72	117	56	48*
			81	103	96	40	48*
		350	59	75	77	44	46
			62	83	88	47	46
		550	72	79	114	53	59*
			58	77	112	65	59*
		750	71	42	114	54	55*
			63	33	106	57	55*
		950	91	34	115	42	45
			79	20	115	48	45
Positive Control	3-Methylcholanthrene	2.5	62	51	520	280	257*
Positive Control			65	52	456	234	257*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	105	139	64	66
			79	92	160	68
			75	107	166	74
			74	96	130	58
Test Chemical	Monuron	300	95	136	173	61	58
			73	88	123	56	58
		500	70	84	158	75	72
			72	74	148	69	72
		700	78	40	178	76	74
			74	42	159	72	74
		900	58	16	117	68	75
			72	12	176	82	75
		1100	60	9	128	71	73
			58	10	131	75	73
Positive Control	3-Methylcholanthrene	2.5	33	22	427	438	406*
Positive Control			41	27	454	374	406*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination