Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02502-0010-0000-2
Chemical Name:	N,N'-Dicyclohexylthiourea
CASRN:	1212-29-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G55329
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	95	82	37	33
Vehicle Control			81	105	69	28	33
Test Chemical	N,N'-Dicyclohexylthiourea	27.7	90	65	32	12	19
			70	53	53	25	19
		50.3	72	35	66	30	28
			77	50	58	25	28
		91.5	72	33	35	16	17
			74	32	41	18	17
		166.4	64	27	59	31	31
			59	21	55	31	31
		302.5	63	19	47	25	21
			64	24	31	16	21
		550	64	14	41	21	20
			68	18	38	19	20
		1000	61	13	56	31	30
			58	12	51	29	30
Positive Control	Ethyl Methane Sulfonate	500	29	21	711	808	856*
Positive Control			27	20	724	905	856*

Nonactivation Trial: 2 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	116	105	172	49	38
			109	106	132	40
			109	97	108	33
			91	92	83	31
Test Chemical	N,N'-Dicyclohexylthiourea	84	91	31	113	41	36
			97	30	73	25
			91	27	116	43
		105	91	31	143	52	54
			80	23	166	70
			87	26	102	39
		131	55	16	127	77	79*
			102	26	244	80	79*
		164	58	12	102	58	82*
			46	13	104	76
			35	6	115	111
Positive Control	Ethyl Methane Sulfonate	500	31	23	1234	1313	893*
			76	53	1298	569
			52	35	1246	796

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	91	93	39	41
			110	104	143	43
			100	105	126	42
Test Chemical	N,N'-Dicyclohexylthiourea	62.9	66	21	119	61	54
			75	25	105	46	54
		78.6	59	17	101	57	58
			63	20	141	74
			70	20	91	43
		98.3	55	14	86	52	60
			62	18	108	58
			69	20	144	70
		122.9	77	14	116	50	49
			81	21	131	54
			90	15	120	44
		153.6	76	14	91	40	42
			74	16	109	49
			73	16	79	36
Positive Control	Ethyl Methane Sulfonate	500	58	37	1151	663	617*
			59	37	869	492
			57	38	1194	696

Non-Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	106	106	41	43
			86	106	116	45
			82	92	98	40
			83	96	118	48
Test Chemical	N,N'-Dicyclohexylthiourea	52	90	116	155	58	54
			77	99	118	51	54
		65	95	92	120	42	50
			82	83	141	58	50
		82	76	39	165	73	81*
			63	32	171	90	81*
		102	67	25	281	140	148*
			68	25	320	156	148*
		128	56#	25	216	128	97*
			59	9	115	65	97*
		160	83	53	131	52	56
			76	45	135	60	56
		200	71	42	116	55	53
			68	43	104	51	53
Positive Control	3-Methylcholanthrene	5	57	58	400	233	215*
			77	68	430	187
			69	65	462	224

Non-Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	136r	136	214	53	77
			119	106	285	80
			108	96	246	76
			99	97	224	75
Test Chemical	N,N'-Dicyclohexylthiourea	63	88	93	203	77	71
			96	112	188	65	71
		79	80	64	195	82	72
			91	75	171	63	72
		98	28	11	213	254
		123	68	35	203	100
		154	78	42	214	91	102
			61	32	207	113	102
		192	71	38	183	86	92
			72	38	211	98	92
		240	76	39	208	92	87
			79	37	196	83	87
		300	88	45	243	92
			167r	40	159	32
Positive Control	3-Methylcholanthrene	5	94	85	875	310	292*
			98	89	870	295
			116	98	938	271

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102#	109	108	35	42
Vehicle Control			93	91	136	49	42
Test Chemical	N,N'-Dicyclohexylthiourea	140	94	65	133	47	42
			96	60	107	37	42
		233.3	81	44	93	38	38
			92	57	104	38	38
		388.8	84	59	127	50	55
			79	50	142	60	55
		648	71	50	119	56	46
			68	38	72	35	46
		1080	83	54	145	58	50
			97	63	124	43	50
		1800	89	42	128	48	60
			72	41	155	72	60
		3000	86	42	123	48	44
			91	53	110	40	44
Positive Control	3-Methylcholanthrene	5	64	33	335	175	215*
Positive Control			41	17	311	255	215*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	85	249	83	65
			118	97	215	61
			108	91	211	65
			112	126	175	52
Test Chemical	N,N'-Dicyclohexylthiourea	52	96	84	213	74	67
			106	59	178	56
			91	71	195	72
		66	83	33	196	79	77
			99	57	233	79
			81	48	178	74
		82	91	41	201	74	82
			103	36	251	82
			79	42	212	90
		102	90	39	219	81	86
			87	41	232	89
			88	42	236	89
		128	95	43	235	83	78
			96	46	184	64
			90	39	235	87
		160	85	40	234	92	78
			106#	87	181.5	57
			95	69	237	83
		200	93	63	210	75	70
			108	89	213	66
			104	71	217	70
Positive Control	3-Methylcholanthrene	5	86	71	554	214	224*
			84#	77	515	205
			85	66	644	254

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination