Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02504-0009-0000-2
Chemical Name:	N,N'-Diethylthiourea
CASRN:	105-55-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	142424
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58	115	176	101	101
			61#	106	192	105
			79#	79	231	98
Test Chemical	N,N'-Diethylthiourea	600	84	104	205	82	84
			82	104	210	86	84
		1100	96#	77	363	126	130
			85#	65	340.5	134	130
		1600	68#	43	1344	657	628*
			71#	40	1276.5	599	628*
		2100	31	16	2487	2689	2408*
			42	18	2660	2128	2408*
		2600	31	9	1842	2002
Positive Control	Methyl Methane Sulfonate	15	34#	34	262.5	259	260*
Positive Control			36#	28	283.5	261	260*

Nonactivation Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	93	70	33	40
			97	101	106	36
			85	108	96	38
			69	99	110	53
Test Chemical	N,N'-Diethylthiourea	500	64	95	77	40	37
			72	83	75	35	37
		1000	76	54	105	46	46
			95	64	129	45	46
		1500	73	15	326	148	182*
			72	15	468	216	182*
		2000	63	7	868	460	600*
			50	6	1105	739	600*
		2500

Positive Control	Methyl Methane Sulfonate	15	42	37	222	177	177*
Positive Control			51	32	269	176	177*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination