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Study Information
Study Attribute Value
CEBS Accession Number: 002-02505-0001-0000-5
Chemical Name: N,N'-Diphenyl-p-phenylenediamine
CASRN: 74-31-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 494928_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 11/12/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 451 0.431 9.02 26.0 0.000
Test Chemical N,N'-Diphenyl-p-phenylenediamine 
 0.16      
 50 1050 535 0.510 10.70 26.0 18.286 
 0.5       
 50 1049 587 0.560 11.74 26.0 29.907 
1.6       
 50 1050 590 0.562 11.80 26.0 30.447 
5         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1048 624 0.595 12.48 26.0 38.227
Mitomycin-C 
 0.005     
 10 210 268 1.276 26.80 26.0 196.269
Trend: 4.465        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/17/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 421 0.401 8.42 26.0 0.000
Test Chemical N,N'-Diphenyl-p-phenylenediamine 
 0.5       
 50 1047 457 0.436 9.14 26.0 8.862 
1.6       
 50 1047 543 0.519 10.86 26.0 29.348 
3         
 50 1046 694 0.663 13.88 31.0 65.476 
4         
 50 1048 718 0.685 14.36 31.0 70.872
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 631 0.601 12.62 26.0 49.881
Mitomycin-C 
 0.005     
 10 211 370 1.754 37.00 26.0 337.347
Trend: 10.985        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 11/12/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 452 0.432 9.04 26.0 0.000
Test Chemical N,N'-Diphenyl-p-phenylenediamine 
1.6       
 50 1044 498 0.477 9.96 26.0 10.494 
5         
 50 1046 470 0.449 9.40 26.0 4.082 
16         
 50 1048 577 0.551 11.54 26.0 27.533 
50         
 50 1047 665 0.635 13.30 26.0 47.124
Positive Control Cyclophosphamide 
 0.15      
 50 1041 832 0.799 16.64 26.0 85.132
Cyclophosphamide 
 0.6       
 10 212 290 1.368 29.00 26.0 216.862
Trend: 6.897        
Probability: 0.000