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Study Information
Study Attribute Value
CEBS Accession Number: 002-02506-0001-0000-6
Chemical Name: N,N'-bis(1,4-Dimethylpentyl)-p-phenylenediamine
CASRN: 3081-14-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 388718_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Test Failure 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 02/06/1986    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 431 0.410 8.62 26.0 0.000
Test Chemical N,N'-bis(1,4-Dimethylpentyl)-p-phenylenediamine 
 0.16      
 50 1050 447 0.426 8.94 26.0 3.712 
 0.5       
 50 1049 495 0.472 9.90 26.0 14.959 
1.6       
 50 1049 489 0.466 9.78 26.0 13.565 
5         
 50 1050 548 0.522 10.96 26.0 27.146 
16         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1051 581 0.553 11.62 26.0 34.675
Mitomycin-C 
 0.005     
 10 210 282 1.343 28.20 26.0 227.146
Trend: 3.978        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 05/01/1986    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 467 0.445 9.34 26.0 0.000
Test Chemical N,N'-bis(1,4-Dimethylpentyl)-p-phenylenediamine 
 0.5       
 50 1049 446 0.425 8.92 26.0 -4.406 
1.6       
 50 1047 497 0.475 9.94 26.0 6.729 
2.5       
 50 1048 530 0.506 10.60 26.0 13.707 
5         
 50 1049 477 0.455 9.54 26.0 2.239 
7.5       
 0 0 0 0.000 0.00 31.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 626 0.597 12.52 26.0 34.175
Mitomycin-C 
 0.005     
 10 210 327 1.557 32.70 26.0 250.107
Trend: 1.666        
Probability: 0.048        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/01/1986    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 489 0.467 9.78 26.0 0.000
Test Chemical N,N'-bis(1,4-Dimethylpentyl)-p-phenylenediamine 
 0.5       
 50 1047 543 0.519 10.86 26.0 11.043 
1.6       
 50 1048 554 0.529 11.08 26.0 13.184 
5         
 50 1044 546 0.523 10.92 26.0 11.977 
16         
 50 1038 575 0.554 11.50 26.0 18.606
Positive Control Cyclophosphamide 
 0.1       
 50 1046 583 0.557 11.66 26.0 19.337
Cyclophosphamide 
 0.6       
 10 210 238 1.133 23.80 26.0 142.658
Trend: 2.531        
Probability: 0.006        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 06/02/1987    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 468 0.446 9.36 26.0 0.000
Test Chemical N,N'-bis(1,4-Dimethylpentyl)-p-phenylenediamine 
 0.16      
 50 1052 461 0.438 9.22 26.0 -1.777 
 0.5       
 50 1050 444 0.423 8.88 26.0 -5.219 
1.6       
 50 1048 409 0.390 8.18 26.0 -12.523 
5         
 50 1050 519 0.494 10.38 26.0 10.792
Positive Control Cyclophosphamide 
 0.1       
 50 1049 563 0.537 11.26 26.0 20.299
Cyclophosphamide 
 0.6       
 10 210 227 1.081 22.70 26.0 142.290
Trend: 0.746        
Probability: 0.228