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Study Information
Study Attribute Value
CEBS Accession Number: 002-02507-0001-0000-7
Chemical Name: N,N'-methylenebisacrylamide
CASRN: 110-26-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 878009_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 04/06/1989    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 349 0.335 6.98 26.0 -16.227
Dimethyl Sulfoxide 
0         
 50 1044 417 0.399 8.34 31.0 0.000
Test Chemical N,N'-methylenebisacrylamide 
2.5       
 50 1049 413 0.394 8.26 26.0 -1.431 
8.3       
 50 1036 487 0.470 9.74 26.0 17.688 
25         
 50 1038 482 0.464 9.64 26.0 16.256 
83         
 50 1582 774 0.489 15.48 31.0 22.490 
250         
 0 0 0 0.000 0.00 0.0 N/A 
833         
 0 0 0 0.000 0.00 0.0 N/A 
2500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1045 688 0.658 13.76 26.0 64.830
Mitomycin-C 
 0.005     
 10 208 253 1.216 25.30 26.0 204.524
Trend: 4.093        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 06/13/1989    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 413 0.395 8.26 31.0 0.000
Dimethyl Sulfoxide 
0         
 50 1035 436 0.421 8.72 26.0 6.589
Test Chemical N,N'-methylenebisacrylamide 
20         
 50 1039 496 0.477 9.92 26.0 20.790 
40         
 50 1038 528 0.509 10.56 26.0 28.707 
60         
 50 1044 530 0.508 10.60 31.0 28.452 
80         
 50 1315 712 0.541 14.24 31.0 37.000 
100         
 0 0 0 0.000 0.00 0.0 N/A 
120         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1052 621 0.590 12.42 26.0 49.363
Mitomycin-C 
 0.005     
 10 210 280 1.333 28.00 26.0 237.369
Trend: 4.852        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/06/1989    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1048 427 0.407 8.54 26.0 -2.733
Dimethyl Sulfoxide 
0         
 50 1048 439 0.419 8.78 31.0 0.000
Test Chemical N,N'-methylenebisacrylamide 
25         
 50 1045 418 0.400 8.36 26.0 -4.510 
83         
 50 1042 410 0.393 8.20 26.0 -6.068 
250         
 50 1049 472 0.450 9.44 26.0 7.415 
833         
 50 1844 1028 0.557 20.56 31.0 33.085 
2500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.125     
 50 1053 567 0.538 11.34 26.0 28.544
Cyclophosphamide 
 0.5       
 10 210 184 0.876 18.40 26.0 109.168
Trend: 6.309        
Probability: 0.000