COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02509-0001-0000-9
Chemical Name: N,N,N',N'-Tetramethyl-p-phenylenediamine
CASRN: 100-22-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 340355_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 10/22/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1053 447 0.425 8.94 26.0 0.000
Test Chemical N,N,N',N'-Tetramethyl-p-phenylenediamine 
 0.5       
 50 1049 531 0.506 10.62 26.0 19.245 
1.6       
 50 1048 579 0.552 11.58 26.0 30.148 
5         
 50 1047 665 0.635 13.30 26.0 49.622 
16         
 50 1048 760 0.725 15.20 26.0 70.834 
50         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 584 0.556 11.68 26.0 31.022
Mitomycin-C 
 0.005     
 10 210 265 1.262 26.50 26.0 197.267
Trend: 9.924        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/17/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 417 0.397 8.34 26.0 0.000
Test Chemical N,N,N',N'-Tetramethyl-p-phenylenediamine 
1.6       
 50 1047 464 0.443 9.28 26.0 11.590 
5         
 50 1047 514 0.491 10.28 26.0 23.615 
16         
 50 1046 739 0.707 14.78 26.0 77.896 
30         
 36 739 539 0.729 14.97 26.0 83.653
Positive Control Mitomycin-C 
 0.0E-4    
 50 1042 557 0.535 11.14 26.0 34.599
Mitomycin-C 
 0.005     
 10 210 267 1.271 26.70 26.0 220.144
Trend: 12.251        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/22/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 411 0.392 8.22 26.0 0.000
Test Chemical N,N,N',N'-Tetramethyl-p-phenylenediamine 
1.6       
 50 1049 472 0.450 9.44 26.0 14.732 
5         
 50 1050 481 0.458 9.62 26.0 16.809 
16         
 50 1050 490 0.467 9.80 26.0 18.994 
50         
 50 1050 752 0.716 15.04 26.0 82.620
Positive Control Cyclophosphamide 
 0.15      
 50 1048 734 0.700 14.68 26.0 78.589
Cyclophosphamide 
 0.6       
 10 210 381 1.814 38.10 26.0 362.621
Trend: 9.657        
Probability: 0.000