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Study Information
Study Attribute Value
CEBS Accession Number: 002-02509-0002-0000-0
Chemical Name: N,N,N',N'-Tetramethyl-p-phenylenediamine
CASRN: 100-22-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 340355_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive* 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #:1   Activation: No Activation    Date: 12/30/1985    Harvest Time: 12.5 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: N,N,N',N'-Tetramethyl-p-phenylenediamine 
16         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
30         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
50         
 200 17 0.085 6.5 1 0.005 0.5 16 0.080 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 40 0.200 15.5 17 0.085 7.5 23 0.115 9.5 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 12 0.240 22.0 8 0.160 16.0 4 0.080 8.0 0 0.000 0.0
Trend: 3.705 1.305 3.485  
Probability: 0.000 0.096 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/09/1986    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Test Chemical: N,N,N',N'-Tetramethyl-p-phenylenediamine 
50         
 200 14 0.070 7.0 6 0.030 3.0 8 0.040 4.0 0 0.000 0.0 
75         
 200 12 0.060 5.5 2 0.010 0.5 10 0.050 5.0 0 0.000 0.0 
100         
 200 27 0.135 9.5 12 0.060 3.5 15 0.075 7.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 31 0.155 15.0 6 0.030 3.0 25 0.125 12.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 15 0.300 28.0 3 0.060 6.0 12 0.240 24.0 0 0.000 0.0
Trend: 2.953 1.380 2.962  
Probability: 0.002 0.084 0.002  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 12/30/1985    Harvest Time: 13.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: N,N,N',N'-Tetramethyl-p-phenylenediamine 
30         
 200 6 0.030 2.5 3 0.015 1.0 3 0.015 1.5 0 0.000 0.0 
50         
 200 19 0.095 5.5 12 0.060 3.5 7 0.035 2.5 0 0.000 0.0 
100         
 200 71 0.355 20.0 39 0.195 12.0 30 0.150 10.5 2 0.010 2.0
Positive Control: Cyclophosphamide 
5         
 200 58 0.290 23.0 19 0.095 7.0 39 0.195 19.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 18 0.360 28.0 4 0.080 6.0 14 0.280 24.0 0 0.000 0.0
Trend: 7.725 6.236 5.295  
Probability: 0.000 0.000 0.000