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Study Information
Study Attribute Value
CEBS Accession Number: 002-02516-0001-0000-7
Chemical Name: N,N-Dimethyl-p-phenylenediamine
CASRN: 99-98-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 372778_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 10/22/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1053 447 0.425 8.94 26.0 0.000
Test Chemical N,N-Dimethyl-p-phenylenediamine 
 0.16      
 50 1048 568 0.542 11.36 26.0 27.676 
 0.5       
 50 1049 507 0.483 10.14 26.0 13.855 
1.6       
 50 1046 639 0.611 12.78 26.0 43.910 
5         
 50 1052 722 0.686 14.44 26.0 61.675 
16         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 584 0.556 11.68 26.0 31.022
Mitomycin-C 
 0.005     
 10 210 265 1.262 26.50 26.0 197.267
Trend: 8.207        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/12/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 438 0.418 8.76 26.0 0.000
Test Chemical N,N-Dimethyl-p-phenylenediamine 
 0.5       
 50 1046 502 0.480 10.04 26.0 14.831 
1.6       
 50 1045 529 0.506 10.58 26.0 21.123 
5         
 50 1044 644 0.617 12.88 26.0 47.595 
10         
 50 1046 791 0.756 15.82 26.0 80.939
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 620 0.590 12.40 26.0 41.283
Mitomycin-C 
 0.005     
 10 211 322 1.526 32.20 26.0 265.141
Trend: 10.943        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/22/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 411 0.392 8.22 26.0 0.000
Test Chemical N,N-Dimethyl-p-phenylenediamine 
5         
 50 1051 493 0.469 9.86 26.0 19.609 
16         
 50 1050 558 0.531 11.16 26.0 35.508 
50         
 50 1051 606 0.577 12.12 26.0 47.024 
160         
 50 1047 753 0.719 15.06 26.0 83.387
Positive Control Cyclophosphamide 
 0.15      
 50 1048 734 0.700 14.68 26.0 78.589
Cyclophosphamide 
 0.6       
 10 210 381 1.814 38.10 26.0 362.621
Trend: 10.634        
Probability: 0.000