Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02520-0008-0000-9
Chemical Name:	N,N-Dimethylaniline
CASRN:	121-69-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	365373
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	86	97	62	24	25
			98	86	83	28
			77	105	53	23
			99	111	74	25
Test Chemical	N,N-Dimethylaniline	0.2	88	51	70	27	28
			80	47	51	21
			69	35	78	37
		0.3	70	23	79	38	37
			72	27	92	42
			69	33	62	30
		0.4	60	9	92	51	53*
			75	17	79	35
			40	3	89	74
		0.5					40*
			61	12	68	37
			73	6	96	44
		0.6


Positive Control	Methyl Methane Sulfonate	5	41	35	392	323	282*
Positive Control			58	41	421	241	282*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	90	89	107	40	43
			65	99	83	43
			68	111	96	47
			82	101	104	42
Test Chemical	N,N-Dimethylaniline	0.2	66	69	80	41	30
			58	76	54	31
			74	86	41	18
		0.3	72	53	95	44	40
			80	66	96	40
			66	51	72	37
		0.4	81	71	78	32	41
			79	35	133	56
			91	62	96	35
		0.5	58	32	87	50	59
			66	30	120	61
			82	15	162	66
		0.6	81	33	162	67	70*
			75	23	171	76
			62	21	127	68
		0.8

		1


Positive Control	Methyl Methane Sulfonate	5	82	73	567	232	265*
Positive Control			62	55	556	297	265*

Induced S9 Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	60	115	261	144	149


			64	85	294	153
Test Chemical	N,N-Dimethylaniline	3	52	106	281	181	168
			59	60	287	162
			54	88	262	162
		5	57	77	372	218	236
			54	31	410	254	236
		8	70	106	385	183	327*
			50	33	805	533
			54	77	427	265
		10	70	106	471	226	241*
			79	129	537	228
			59	70	477	269
Positive Control	3-Methylcholanthrene	1.25					475*
			72	66	774	360
			56	24	981	589

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	87	99	129	50	47
			93	103	140	50
			88	77	106	40
			115	121	164	48
Test Chemical	N,N-Dimethylaniline	10	77	84	133	57	63
			59	76	135	76
			87	107	140	54
		20	64	84	156	82	70*
			81	80	162	67
			101	92	184	61
		30	101	85	234	77	86*
			62	55	181	97
			79	100	199	84
		40	73	66	236	108	121*
			71	46	237	111
			74	67	321	144
		50	86	23	512	199	175*
			74	45	424	191
			91	81	365	134
		60	69	35	382	185	181*
			77	49	386	166
			60	32	340	190
Positive Control	3-Methylcholanthrene	2.5	58#	31	819	475	445*
			77	43	842	367
			54	28	804	493

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	81	95	196	81	66
			80	87	153	64
			93	94	172	62
			114	124	194	57
Test Chemical	N,N-Dimethylaniline	20	66#	60	244	124	177*
			52	45	381	244
			66	58	319	162
		30	70#	27	529	251	276*
			75	31	621	278
			53	26	477	299
		40	56	10	1020	607	566*
			38	6	662	581
			55	18	836	511
		50					622*
			48	6	507	351
			34#	3	912	894
		60					736*
			44	6	859	653
			29	4	708	818
		80


Positive Control	3-Methylcholanthrene	2.5	20	4	547	897	889*
			25	4	623	848
			24	10	668	921

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination