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Study Information
Study Attribute Value
CEBS Accession Number: 002-02520-0008-0000-9
Chemical Name: N,N-Dimethylaniline
CASRN: 121-69-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 365373
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 86 97 62 24 25
    98 86 83 28
    77 105 53 23
    99 111 74 25
Test Chemical N,N-Dimethylaniline 
 0.2       
 88 51 70 27 28
    80 47 51 21
    69 35 78 37
  
 0.3       
 70 23 79 38 37
    72 27 92 42
    69 33 62 30
  
 0.4       
 60 9 92 51 53*
    75 17 79 35
    40 3 89 74
  
 0.5       
 40*
    61 12 68 37
    73 6 96 44
  
 0.6       
   
   
Positive Control Methyl Methane Sulfonate 
5         
 41 35 392 323 282*
    58 41 421 241

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 90 89 107 40 43
    65 99 83 43
    68 111 96 47
    82 101 104 42
Test Chemical N,N-Dimethylaniline 
 0.2       
 66 69 80 41 30
    58 76 54 31
    74 86 41 18
  
 0.3       
 72 53 95 44 40
    80 66 96 40
    66 51 72 37
  
 0.4       
 81 71 78 32 41
    79 35 133 56
    91 62 96 35
  
 0.5       
 58 32 87 50 59
    66 30 120 61
    82 15 162 66
  
 0.6       
 81 33 162 67 70*
    75 23 171 76
    62 21 127 68
  
 0.8       
   
  
1         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 82 73 567 232 265*
    62 55 556 297

Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 60 115 261 144 149
   
   
    64 85 294 153
Test Chemical N,N-Dimethylaniline 
3         
 52 106 281 181 168
    59 60 287 162
    54 88 262 162
  
5         
 57 77 372 218 236
    54 31 410 254
  
8         
 70 106 385 183 327*
    50 33 805 533
    54 77 427 265
  
10         
 70 106 471 226 241*
    79 129 537 228
    59 70 477 269
Positive Control 3-Methylcholanthrene 
1.25      
 475*
    72 66 774 360
    56 24 981 589

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 87 99 129 50 47
    93 103 140 50
    88 77 106 40
    115 121 164 48
Test Chemical N,N-Dimethylaniline 
10         
 77 84 133 57 63
    59 76 135 76
    87 107 140 54
  
20         
 64 84 156 82 70*
    81 80 162 67
    101 92 184 61
  
30         
 101 85 234 77 86*
    62 55 181 97
    79 100 199 84
  
40         
 73 66 236 108 121*
    71 46 237 111
    74 67 321 144
  
50         
 86 23 512 199 175*
    74 45 424 191
    91 81 365 134
  
60         
 69 35 382 185 181*
    77 49 386 166
    60 32 340 190
Positive Control 3-Methylcholanthrene 
2.5       
 58# 31 819 475 445*
    77 43 842 367
    54 28 804 493

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 81 95 196 81 66
    80 87 153 64
    93 94 172 62
    114 124 194 57
Test Chemical N,N-Dimethylaniline 
20         
 66# 60 244 124 177*
    52 45 381 244
    66 58 319 162
  
30         
 70# 27 529 251 276*
    75 31 621 278
    53 26 477 299
  
40         
 56 10 1020 607 566*
    38 6 662 581
    55 18 836 511
  
50         
 622*
    48 6 507 351
    34# 3 912 894
  
60         
 736*
    44 6 859 653
    29 4 708 818
  
80         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 20 4 547 897 889*
    25 4 623 848
    24 10 668 921

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination