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Study Information
Study Attribute Value
CEBS Accession Number: 002-02521-0002-0000-4
Chemical Name: N,N-Dimethylurea
CASRN: 96-31-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 206480
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 109 109 107 33 36
    102 104 78 25
    102 95 154 50
    78 91 84 36
Test Chemical N,N-Dimethylurea 
500         
 78 70 79 34 35
    94 83 77 27
    102 73 130 42
  
1000         
 81 67 95 39 31
    80 76 60 25
    101 81 89 29
  
2000         
 93 71 88 31
    129r 81 116 30
    122r 88 115 31
  
3000         
 133r 71 132 33 40
    119 74 142 40
    79 79 97 41
  
4000         
 94 94 112 40 40
    88# 70 112 42
    120 66 134 37
  
5000         
 118 78 128 36 39
    99 63 126 43
    133r 63 159 40
Positive Control Methyl Methane Sulfonate 
5         
 94 46 657 233 224*
    72 53 528 244
    69 37 400 194

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 104 94 80 26 23
    124r 117 65 18
    101 80 78 26
    111# 126 58 17
Test Chemical N,N-Dimethylurea 
500         
 115 92 87 25 25
    104# 178 73 23
    74 40 60 27
  
1000         
 82 76 81 33 27
    114 68 56 16
    78 100 77 33
  
2000         
 100 120 58 19 18
    101 47 46 15
    86 53 52 20
  
3000         
 63 73 41 22 22
    88 94 75 28
    108 108 54 17
  
4000         
 114 116 87 26 25
    102# 101 68 22
    104 93 85 27
  
5000         
 108 85 91 28 27
    100 88 92 31
    95 36 64 22
Positive Control Methyl Methane Sulfonate 
5         
 113# 60 276 82 97*
    82 44 306 124
    111 62 284 85

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 101 101 84 28 29
    115 104 86 25
    112 102 85 25
    101 93 111 37
Test Chemical N,N-Dimethylurea 
500         
 112 74 92 27 34
    82 58 101 41
  
1000         
 86 54 87 34 29
    97 66 93 32
    110 80 73 22
  
2000         
 94 64 97 35 33
    97 72 81 28
    80 70 91 38
  
3000         
 113 56 108 32 30
    102 76 93 30
    89 66 72 27
  
4000         
 93 56 85 31 31
    89 57 83 31
    102 68 97 32
  
5000         
 85 43 163 64 52*
    115 84 179 52
    119 60 144 40
Positive Control 3-Methylcholanthrene 
2.5       
 94 66 402 142 183*
    120 52 749 209
    101 51 595 197

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 70 102 96 46 43
    78 69 107 46
    80 103 108 45
    72 127 77 36
Test Chemical N,N-Dimethylurea 
500         
 69 135 69 34 38
    66 107 81 41
    86 127 99 39
  
1000         
 89 117 96 36 46
    69 78 99 48
    65 85 104 53
  
2000         
 68 60 125 62 48
    75 117 89 39
    65# 123 86 44
  
3000         
 88 103 121 46 55
    54 65 103 64
    51 52 83 54
  
4000         
 78 71 104 45 51
    63 62 115 61
    75 104 103 46
  
5000         
 71 99 143 67 56
    67 71 99 49
    59 75 94 53
Positive Control 3-Methylcholanthrene 
2.5       
 84 85 480 191 213*
    72 87 445 206
    65 51 471 243

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 58 35 154 88 86
    76 142 137 60
    78 94 260 111
    99 129 257 87
Test Chemical N,N-Dimethylurea 
500         
 67 39 182 90 72
    85 105 146 57
    77 82 157 68
  
1000         
 95 60 194 68 72
    85 106 148 58
    71 48 191 89
  
2000         
 88# 84 190 72 69
    89# 100 174 65
  
3000         
 113# 142 220 65 74
    104 116 258 83
    72 57 159 74
  
4000         
 80 44 204 86 87
    73 66 192 87
    74 55 196 88
  
5000         
 118 79 238 67 70
    88 125 191 72
    120r 76 250 69
Positive Control 3-Methylcholanthrene 
2.5       
 54 35 991 614 645*
    82 54 1402 572
    62# 39 1400 749

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination