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Study Information
Study Attribute Value
CEBS Accession Number: 002-02524-0009-0000-4
Chemical Name: N-(1-Naphthyl)ethylenediamine dihydrochloride
CASRN: 1465-25-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 356358
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 94 119 47 41
    97 104 126 44
    88 100 77 29
    97 102 123 42
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
3.75      
 104 97 152 49 41
    92 82 93 34
  
7.5       
 112 85 131 39 39
    102 79 117 38
  
15         
 75 78 87 39 37
    94 76 97 34
  
30         
 79 71 75 32 36
    98 50 118 40
  
60         
 71 13 133 63 48
    78 55 76 32
Positive Control Methyl Methane Sulfonate 
15         
 39 23 255 218 211*
    42 29 257 203

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 64 86 122 63 52
    67 112 79 39
    55 94 93 57
    62 108 87 47
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
20         
 45 64 54 40 33
    46 62 37 27
  
30         
 47 55 73 52 48
    45 38 59 44
  
40         
 40 28 52 44 40
    55 40 59 36
  
50         
 53 13 123 78 63
    26 12 38 48
  
60         
   
Positive Control Methyl Methane Sulfonate 
15         
 17 17 373 731 686*
    18 23 349 640

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 112 136 64 65
    70 92 148 70
    85 88 143 56
    81 108 171 71
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
15         
 86 103 94 37 42
    92 110 132 48
  
25         
 84 74 83 33 34
    80 89 84 35
  
35         
 76 63 102 45 44
    87 69 114 44
  
45         
 104 37 135 43 36
    77 38 66 29
  
55         
 77 10 112 48 48
    70 10 101 48
  
65         
   
Positive Control Methyl Methane Sulfonate 
15         
 33 26 480 482 493*
    34 24 507 504

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 111 76 32 35
    77 102 103 45
    81 107 63 26
    71 80 82 38
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
25         
 58 63 105 61 59*
    58 66 99 57
  
35         
 60 70 139 77 70*
    65 67 122 63
  
45         
 46 56 90 65 68*
    51 59 107 71
  
55         
 67 41 134 66 63*
    51 40 90 59
  
65         
 63 20 144 76 60*
    91 24 122 45
Positive Control 3-Methylcholanthrene 
2.5       
 39 18 346 293 330*
    38 20 417 366

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 81 95 144 59 47
    103 100 119 39
    97 97 142 49
    111 108 142 43
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
35         
 89 76 168 63 55
    91 90 131 48
  
45         
 72 43 170 78 71
    81 84 152 63
  
55         
 88 61 144 55 64
    81 64 177 73
  
65         
 82 46 139 57 55
    102 52 166 54
  
75         
 75 21 176 78 72
    77 21 153 66
Positive Control 3-Methylcholanthrene 
2.5       
 52 36 615 390 394*
    61 52 722 398

Induced S9 Trial: 3 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 57 98 168 99 88
    69 98 223 108
    64# 112 123 64
    56 92 135 80
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
35         
 47 59 124 88
  
45         
 48# 42 103 72 69
    54 59 106 66
  
55         
 53 57 148 94
  
65         
 42 38 102 80 71
    44 36 83 62
  
75         
 65 44 69 36 36
    71# 56 80 37
Positive Control 3-Methylcholanthrene 
2.5       
 21 16 265 421 472*
    19 14 296 524

Induced S9 Trial: 4 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 76 111 31 14 15
    80 92 28 12
    95 90 46 16
    66 108 37 19
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
35         
 88 77 34 13 13
    88 71 33 13
  
45         
 73 67 36 16 14
    111 65 39 12
  
55         
 91 22 34 12 17
    88 43 58 22
  
65         
 79 24 24 10 14
    71 21 40 19
  
75         
 48 14 18 13 19
    75 13 57 25
  
85         
   
Positive Control 3-Methylcholanthrene 
2.5       
 45 25 230 170 151*
    42 25 165 131

Induced S9 Trial: 5 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 56 83 65 39 33
    75 82 93 41
    81 137 77 32
    67 98 40 20
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
55         
 70 60 26 12 21
    89 85 79 30
  
65         
 71 53 44 21 25
    85 55 76 30
  
75         
 75 35 43 19 26
    75 47 74 33
  
85         
 79 18 70 30 31
    80 21 76 32
  
95         
 59 12 58 33 43
    72 14 115 54
Positive Control 3-Methylcholanthrene 
2.5       
 70 44 448 212 231*
    61 38 456 251

Induced S9 Trial: 6 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 107 60 25 27
    95 107 71 25
    93# 96 70 25
    71 90 67 32
Test Chemical N-(1-Naphthyl)ethylenediamine dihydrochloride 
35         
 66 81 70 36 31
    72 102 58 27
  
45         
 72 64 61 28 37
    64 62 87 45
  
55         
 65 39 65 34 35
    67 46 74 37
  
65         
 57 26 55 32 40
    69 28 99 48
  
75         
 54 10 73 45 39
    65 13 64 33
  
85         
   
Positive Control 3-Methylcholanthrene 
2.5       
 24 11 361 512 458*
    24 11 287 404

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination