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Study Information
Study Attribute Value
CEBS Accession Number: 002-02542-0002-0000-7
Chemical Name: N-Isopropyl-N'-phenyl-p-phenylenediamine
CASRN: 101-72-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 042763_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 03/19/1986    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: N-Isopropyl-N'-phenyl-p-phenylenediamine 
1.6       
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
3         
 200 15 0.075 5.5 5 0.025 2.0 10 0.050 3.5 0 0.000 0.0 
5         
 200 14 0.070 6.0 2 0.010 1.0 12 0.060 6.0 0 0.000 0.0 
10         
 55 9 0.164 10.9 3 0.055 5.5 6 0.109 9.1 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 200 57 0.285 22.0 16 0.080 7.5 41 0.205 16.5 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 22 0.440 20.0 4 0.080 6.0 8 0.160 12.0 10 0.200 1.0
Trend: 4.057 3.086 3.770  
Probability: 0.000 0.001 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/22/1986    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0
Test Chemical: N-Isopropyl-N'-phenyl-p-phenylenediamine 
10         
 200 28 0.140 5.5 7 0.035 1.0 11 0.055 4.5 10 0.050 1.0 
16         
 200 19 0.095 8.0 6 0.030 3.0 13 0.065 5.5 0 0.000 0.0 
30         
 200 15 0.075 5.0 9 0.045 3.0 6 0.030 3.0 0 0.000 0.0 
50         
 134 16 0.119 9.0 2 0.015 1.5 13 0.097 6.7 1 0.007 1.0
Positive Control: Cyclophosphamide 
5         
 200 39 0.195 17.5 6 0.030 3.0 33 0.165 15.5 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 20 0.400 32.0 2 0.040 4.0 18 0.360 30.0 0 0.000 0.0
Trend: 2.279 1.865 1.443  
Probability: 0.011 0.031 0.074  


Trial #:2   Activation: No Activation    Date: 04/22/1986    Harvest Time: 17.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: N-Isopropyl-N'-phenyl-p-phenylenediamine 
3         
 200 11 0.055 5.5 1 0.005 0.5 10 0.050 5.0 0 0.000 0.0 
5         
 200 90 0.450 19.0 22 0.110 7.0 38 0.190 14.0 30 0.150 3.0 
7.5       
 200 98 0.490 18.0 29 0.145 9.0 39 0.195 14.0 30 0.150 3.0 
10         
 200 60 0.300 14.0 26 0.130 7.0 34 0.170 11.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 59 0.295 25.0 25 0.125 11.5 34 0.170 15.5 0 0.000 0.0
Mitomycin-C 
 0.125     
 200 134 0.670 51.5 60 0.300 26.0 74 0.370 32.0 0 0.000 0.0
Trend: 6.088 4.983 5.182  
Probability: 0.000 0.000 0.000