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Study Information
Study Attribute Value
CEBS Accession Number: 002-02562-0001-0000-8
Chemical Name: N-Nitrosodiphenylamine
CASRN: 86-30-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 672479_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 04/16/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 416 0.402 8.32 26.5 0.000
Test Chemical N-Nitrosodiphenylamine 
1.21      
 50 1031 535 0.519 10.70 26.5 29.229 
4.04      
 50 1035 565 0.546 11.30 26.5 35.949 
12.1       
 50 1037 575 0.554 11.50 26.5 38.088 
40.4       
 50 1031 622 0.603 12.44 26.5 50.244 
121         
 0 0 0 0.000 0.00 30.5 N/A
Positive Control Mitomycin-C 
 0.002     
 50 1032 693 0.672 13.86 26.5 67.232
Mitomycin-C 
 0.01      
 10 207 235 1.135 23.50 26.5 182.725
Trend: 6.173        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 04/29/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1025 372 0.363 7.44 26.5 0.000
Test Chemical N-Nitrosodiphenylamine 
9.4       
 50 1033 475 0.460 9.50 26.5 26.699 
18.8       
 50 1040 537 0.516 10.74 26.5 42.273 
28.2       
 50 1035 622 0.601 12.44 26.5 65.589 
37.6       
 30 618 438 0.709 14.60 30.5 95.284
Positive Control Mitomycin-C 
 0.002     
 50 1036 585 0.565 11.70 26.5 55.588
Mitomycin-C 
 0.01      
 10 208 222 1.067 22.20 26.5 194.083
Trend: 10.289        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/16/1986    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 512 0.492 10.24 26.0 0.000
Test Chemical N-Nitrosodiphenylamine 
12.1       
 50 1020 521 0.511 10.42 26.0 3.753 
40.4       
 50 1020 552 0.541 11.04 26.0 9.926 
121         
 50 1026 752 0.733 15.04 26.0 48.879 
404         
 0 0 0 0.000 0.00 30.0 N/A
Positive Control Cyclophosphamide 
 0.5       
 50 1022 614 0.601 12.28 26.0 22.034
Cyclophosphamide 
2.5       
 10 210 226 1.076 22.60 26.0 118.601
Trend: 7.081        
Probability: 0.000