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Study Information
Study Attribute Value
CEBS Accession Number: 002-02562-0008-0000-5
Chemical Name: N-Nitrosodiphenylamine
CASRN: 86-30-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 114324
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 110 83 92 28 29
    102# 104 105 34
    103 104 69 22
    105 109 102 32
Test Chemical N-Nitrosodiphenylamine 
3.13      
 104 82 70 22 19
    104 107 54 17
    103 70 54 17
  
6.25      
 96 67 72 25 18
    94 126 46 16
    104# 97 39 13
  
12.5       
 87 66 64 24 21
    107 106 61 19
    98 110 53 18
  
25         
 99 60 91 31 27
    104 95 71 23
    101# 64 80 26
  
50         
 95# 11 114 40 32
    103 17 92 30
    105 21 86 27
  
75         
 79 2 124 52
   
   
  
125         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 101 56 1019 338 324*
    99 66 732 246
    88 48 1030 389

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89# 119 117 44 60
    94 97 206 73
    84 89 154 61
    91 95 174 64
Test Chemical N-Nitrosodiphenylamine 
3.13      
 96# 85 150 52 60
    77# 80 135 59
    79 74 164 69
  
6.25      
 76 92 132 58 57
    78# 88 150 64
    111# 107 162 49
  
12.5       
 90 62 147 54 57
    88 92 159 60
  
25         
 104 81 138 44 52
    86# 51 154.5 60
  
50         
 92 22 213 77 85
    84 22 223 89
    82 40 215 88
Positive Control Ethyl Methane Sulfonate 
250         
 43 35 847 664 585*
    53 34 945 594
    68# 36 1009.5 495

Nonactivation Trial: 3 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 88 106 44 46
    91 98 132 48
    87# 119 87 33
    90 95 159 59
Test Chemical N-Nitrosodiphenylamine 
3.13      
 93# 84 112.5 41 48
    73 70 125 57
    74# 79 105 47
  
6.25      
 73 91 93 42 44
    72# 84 114 53
    109# 108 115.5 35
  
12.5       
 89 63 116 43 44
    86 92 117 46
  
25         
 101 80 101 33 39
    80# 49 109.5 45
  
50         
 82 22 150 61 60
    79 40 145 61
    89 22 154 58
Positive Control Ethyl Methane Sulfonate 
250         
 64# 34 898.5 469 540*
    49 32 819 559
    41 34 724 591

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 64 92 115 60 67
    74 96 176 79
    84 121 139 55
    73 91 164 75
Test Chemical N-Nitrosodiphenylamine 
2.5       
 72 59 203 94 95
    72 58 249 115
    85 67 193 76
  
5         
 71 66 195 92 100*
    83 74 221 89
    62 55 222 120
  
10         
 78 45 320 138 146*
    80 40 378 157
    62# 33 264 143
  
20         
 47 17 419 297 271*
    52 14 463 298
    52 23 337 217
  
30         
 35 9 485 462 431*
    41 8 536 436
    45 8 537 395
  
40         
 32 5 270 283 339*
    48 7 447 309
    49# 5 626 426
  
50         
   
   
Positive Control 3-Methylcholanthrene 
3.5       
 52 67 416 269 264*
    66 70 534 269
    64 71 486 254

Non-Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 69 78 33 35
    106 114 118 37
    111 95 104 31
    84 122 94 37
Test Chemical N-Nitrosodiphenylamine 
10         
 121r 132 106 29 37
    89 55 102 38
    76 73 80 35
  
20         
 74 61 85 38 47
    106# 54 138 43
    83 53 146 59
  
30         
 103 52 145 47 54*
    81 37 163 67
    80 81 114 47
  
50         
 74 32 136 62 86*
    69 28 237 114
    76 40 189 83
  
80         
 73 48 179 81 97*
    81 23 257 106
    84 20 260 104
Positive Control 3-Methylcholanthrene 
3.5       
 81 82 389 160 140*
    112 117 412 123
    88 97 356 135

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 101 86 37 32
    81# 94 73 30
    79 104 79 33
    80 101 63 26
Test Chemical N-Nitrosodiphenylamine 
3.13      
 81 70 65 27 23
    69 84 43 21
    74 92 48 22
  
6.25      
 76 62 49 21 26
    93 87 76 27
    76 71 67 29
  
12.5       
 101 46 86 28
  
25         
 81 23 97 40 47*
    73 17 156 72
    73 34 68 31
  
50         
 66# 14 138 70 65*
    69 22 128 62
    84 20 159 63
  
75         
 65 16 111 57 68*
    72 14 200 92
    91 24 151 55
Positive Control 3-Methylcholanthrene 
2.5       
 49 52 278 188 185*
    57 67 334 195
    59 62 300 170

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 89 295 112 105
    99 108 298 100
    106 102 328 103
Test Chemical N-Nitrosodiphenylamine 
1.56      
 93 67 178 64 75
    106 100 224 70
    82# 49 222 90
  
3.13      
 124r 77 289.5 78 67
    101# 59 216 72
    102# 63 188 62
  
6.25      
 95# 51 265.5 93
    136r 56 250 61
    125r 49 286.5 77
  
12.5       
 102 31 249 82 106
    100# 23 390 131
    122r 32 358.5 98
  
25         
 119 20 285 80 95
    78 15 257 109
  
50         
 91 12 357 131 110
    83 6 274 110
    86 6 227 88
  
75         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 65 38 1033 531 500*
    73 36 1106 509
    77# 46 1068 461

Induced S9 Trial: 3 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 90 240 91 83
    97 107 233 80
    105 103 243 77
Test Chemical N-Nitrosodiphenylamine 
1.56      
 79# 48 173 73 61
    105 100 175 55
    92 67 153 55
  
3.13      
 121r 76 211.5 58 51
    100# 59 162 54
    100# 62 142 47
  
6.25      
 123r 49 223.5 60
    135r 56 192 47
    93# 50 195 70
  
12.5       
 100 31 175 58 79
    98# 23 295.5 100
    116# 31 274.5 79
  
25         
 119 20 207 58 69
    77 15 187 81
  
50         
 90 12 267 99 78
    82 6 208 85
    87 6 134 52
  
75         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 63 37 699 371 348*
    68 34 742 363
    77# 46 720 312

Induced S9 Trial: 4 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 81 185 73 83
    132r 94 297 75
    94 92 290 102
    104 127 234 75
Test Chemical N-Nitrosodiphenylamine 
2.5       
 100# 83 423 141 121
    115# 106 330 96
    83 78 317 127
  
5         
 129r 91 402 104
    83# 42 375 150
    129r 89 375 97
  
10         
 98# 37 504 171 188*
    147r 68 382 87
    71# 41 431 204
  
20         
 76# 19 537 237 244*
    89 13 665 250
  
30         
 117# 16 528 150
  
50         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 60# 13 742 411 393*
    56 19 631 376

Induced S9 Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 92 161 59 51
    101 127 155 51
    81 80 106 44
    129r 95 163 42
Test Chemical N-Nitrosodiphenylamine 
2.5       
 81 79 193 79 73
    97# 83 244.5 84
    113# 108 192 57
  
5         
 125r 92 232.5 62
    81# 43 210 86
    126r 90 212 56
  
10         
 96# 37 285 99 111*
    143r 69 226 53
    69# 42 251 122
  
20         
 85 13 346 136 134*
    75# 19 296 132
  
30         
 107# 15 270 84
  
50         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 52# 12 435 276 268*
    52 18 402 259

Induced S9 Trial: 6 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 94 108 79 28 30
    77 70 67 29
    106 112 113 36
    94 110 79 28
Test Chemical N-Nitrosodiphenylamine 
30         
 78 44 195 84 86*
    70 40 194 93
    82 68 199 81
  
40         
 91 40 234 86 83*
    83 25 200 80
    75 34 187 83
  
50         
 83 25 221 89 78*
    104 31 220 71
    98# 38 224 76
  
60         
 82 28 184 75 86*
    64 25 185 96
    68 23 179 87
  
80         
 70 21 205 98 78*
    87 26 163 62
    86 21 190 74
Positive Control 3-Methylcholanthrene 
2.5       
 61 39 424 234 193*
    76# 68 383 169
    72 85 381 176

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination