Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-02566-0008-0000-9 |
Chemical Name: | N-Phenyl-2-naphthylamine |
CASRN: | 135-88-6 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 945201 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 80# | 98 | 91 | 38 | 31 |
72 | 84 | 72 | 33 | ||||
83 | 105 | 59 | 24 | ||||
84 | 113 | 71 | 28 | ||||
Test Chemical | N-Phenyl-2-naphthylamine | 6.25 | 70 | 100 | 82 | 39 | 31 |
78 | 113 | 51 | 22 | ||||
12.5 | 87 | 81 | 78 | 30 | 33 | ||
65 | 86 | 69 | 35 | ||||
25 | 61 | 49 | 56 | 30 | 33 | ||
56 | 41 | 59 | 35 | ||||
50 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 48 | 28 | 338 | 235 | 245* |
36 | 20 | 278 | 255 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 55 | 81 | 86 | 52 | 33 |
70 | 101 | 48 | 23 | ||||
70 | 119 | 49 | 23 | ||||
71 | 99 | 68 | 32 | ||||
Test Chemical | N-Phenyl-2-naphthylamine | 8 | 69 | 91 | 35 | 17 | 22 |
59 | 44 | 48 | 27 | ||||
16 | 65 | 35 | 44 | 23 | 23 | ||
73 | 31 | 50 | 23 | ||||
24 | 41 | 25 | 30 | 24 | 28 | ||
49 | 17 | 46 | 31 | ||||
32 | 44 | 8 | 70 | 53 | 45 | ||
53 | 14 | 60 | 38 | ||||
40 | |||||||
48 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 42 | 18 | 138 | 109 | 96* |
26# | 28 | 66 | 83 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 86 | 86 | 82 | 32 | 35 |
86 | 105 | 68 | 26 | ||||
85 | 99 | 111 | 43 | ||||
76 | 110 | 86 | 38 | ||||
Test Chemical | N-Phenyl-2-naphthylamine | 0.5 | 84 | 76 | 126 | 50 | 43 |
78 | 75 | 86 | 37 | ||||
1 | 84 | 74 | 145 | 58 | 45 | ||
76 | 82 | 72 | 31 | ||||
2 | 88 | 68 | 77 | 29 | 30 | ||
87 | 79 | 82 | 32 | ||||
4 | 80 | 47 | 78 | 32 | 31 | ||
80 | 49 | 71 | 30 | ||||
8 | 69 | 31 | 67 | 32 | 45 | ||
71 | 22 | 122 | 57 | ||||
16 | |||||||
Positive Control | 3-Methylcholanthrene | 2.5 | 42 | 16 | 421 | 337 | 350* |
39 | 17 | 419 | 363 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 62 | 107 | 41 | 22 | 27 |
67 | 92 | 63 | 31 | ||||
68 | 88 | 55 | 27 | ||||
61 | 113 | 50 | 27 | ||||
Test Chemical | N-Phenyl-2-naphthylamine | 4 | 67 | 89 | 67 | 34 | 34 |
67 | 95 | 68 | 34 | ||||
7 | 56 | 32 | 68 | 41 | 42 | ||
59 | 51 | 75 | 42 | ||||
10 | 66# | 33 | 124 | 63 | 56* | ||
58 | 39 | 87 | 50 | ||||
13 | 55 | 17 | 109 | 66 | 75* | ||
37 | 12 | 94 | 84 | ||||
16 | 44 | 6 | 125 | 95 | 84* | ||
30 | 10 | 65 | 73 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 28 | 16 | 334 | 398 | 395* |
24 | 14 | 287 | 393 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 81 | 110 | 139 | 57 | 53 |
82 | 111 | 108 | 44 | ||||
65 | 96 | 121 | 62 | ||||
66 | 83 | 98 | 50 | ||||
Test Chemical | N-Phenyl-2-naphthylamine | 1 | 76 | 92 | 102 | 45 | 51 |
84 | 74 | 144 | 57 | ||||
4 | 80 | 72 | 168 | 70 | 67 | ||
65 | 85 | 126 | 64 | ||||
7 | 57 | 46 | 162 | 96 | 83 | ||
67 | 41 | 140 | 70 | ||||
10 | 66 | 24 | 297 | 151 | 136* | ||
59 | 25 | 213 | 120 | ||||
13 | 57 | 9 | 327 | 193 | 193* | ||
59 | 14 | 345 | 194 | ||||
16 | |||||||
Positive Control | 3-Methylcholanthrene | 2.5 | 69 | 84 | 480 | 234 | 233* |
73 | 66 | 507 | 233 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination