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Study Information
Study Attribute Value
CEBS Accession Number: 002-02566-0008-0000-9
Chemical Name: N-Phenyl-2-naphthylamine
CASRN: 135-88-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 945201
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80# 98 91 38 31
    72 84 72 33
    83 105 59 24
    84 113 71 28
Test Chemical N-Phenyl-2-naphthylamine 
6.25      
 70 100 82 39 31
    78 113 51 22
  
12.5       
 87 81 78 30 33
    65 86 69 35
  
25         
 61 49 56 30 33
    56 41 59 35
  
50         
   
Positive Control Methyl Methane Sulfonate 
15         
 48 28 338 235 245*
    36 20 278 255

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 55 81 86 52 33
    70 101 48 23
    70 119 49 23
    71 99 68 32
Test Chemical N-Phenyl-2-naphthylamine 
8         
 69 91 35 17 22
    59 44 48 27
  
16         
 65 35 44 23 23
    73 31 50 23
  
24         
 41 25 30 24 28
    49 17 46 31
  
32         
 44 8 70 53 45
    53 14 60 38
  
40         
  
48         
   
Positive Control Methyl Methane Sulfonate 
15         
 42 18 138 109 96*
    26# 28 66 83

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 86 82 32 35
    86 105 68 26
    85 99 111 43
    76 110 86 38
Test Chemical N-Phenyl-2-naphthylamine 
 0.5       
 84 76 126 50 43
    78 75 86 37
  
1         
 84 74 145 58 45
    76 82 72 31
  
2         
 88 68 77 29 30
    87 79 82 32
  
4         
 80 47 78 32 31
    80 49 71 30
  
8         
 69 31 67 32 45
    71 22 122 57
  
16         
   
Positive Control 3-Methylcholanthrene 
2.5       
 42 16 421 337 350*
    39 17 419 363

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 62 107 41 22 27
    67 92 63 31
    68 88 55 27
    61 113 50 27
Test Chemical N-Phenyl-2-naphthylamine 
4         
 67 89 67 34 34
    67 95 68 34
  
7         
 56 32 68 41 42
    59 51 75 42
  
10         
 66# 33 124 63 56*
    58 39 87 50
  
13         
 55 17 109 66 75*
    37 12 94 84
  
16         
 44 6 125 95 84*
    30 10 65 73
Positive Control 3-Methylcholanthrene 
2.5       
 28 16 334 398 395*
    24 14 287 393

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 81 110 139 57 53
    82 111 108 44
    65 96 121 62
    66 83 98 50
Test Chemical N-Phenyl-2-naphthylamine 
1         
 76 92 102 45 51
    84 74 144 57
  
4         
 80 72 168 70 67
    65 85 126 64
  
7         
 57 46 162 96 83
    67 41 140 70
  
10         
 66 24 297 151 136*
    59 25 213 120
  
13         
 57 9 327 193 193*
    59 14 345 194
  
16         
   
Positive Control 3-Methylcholanthrene 
2.5       
 69 84 480 234 233*
    73 66 507 233

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination