Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02566-0008-0000-9
Chemical Name:	N-Phenyl-2-naphthylamine
CASRN:	135-88-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	945201
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80#	98	91	38	31
			72	84	72	33
			83	105	59	24
			84	113	71	28
Test Chemical	N-Phenyl-2-naphthylamine	6.25	70	100	82	39	31
			78	113	51	22	31
		12.5	87	81	78	30	33
			65	86	69	35	33
		25	61	49	56	30	33
			56	41	59	35	33
		50

Positive Control	Methyl Methane Sulfonate	15	48	28	338	235	245*
Positive Control			36	20	278	255	245*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	55	81	86	52	33
			70	101	48	23
			70	119	49	23
			71	99	68	32
Test Chemical	N-Phenyl-2-naphthylamine	8	69	91	35	17	22
			59	44	48	27	22
		16	65	35	44	23	23
			73	31	50	23	23
		24	41	25	30	24	28
			49	17	46	31	28
		32	44	8	70	53	45
			53	14	60	38	45
		40
		48

Positive Control	Methyl Methane Sulfonate	15	42	18	138	109	96*
Positive Control			26#	28	66	83	96*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	86	82	32	35
			86	105	68	26
			85	99	111	43
			76	110	86	38
Test Chemical	N-Phenyl-2-naphthylamine	0.5	84	76	126	50	43
			78	75	86	37	43
		1	84	74	145	58	45
			76	82	72	31	45
		2	88	68	77	29	30
			87	79	82	32	30
		4	80	47	78	32	31
			80	49	71	30	31
		8	69	31	67	32	45
			71	22	122	57	45
		16

Positive Control	3-Methylcholanthrene	2.5	42	16	421	337	350*
Positive Control			39	17	419	363	350*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	62	107	41	22	27
			67	92	63	31
			68	88	55	27
			61	113	50	27
Test Chemical	N-Phenyl-2-naphthylamine	4	67	89	67	34	34
			67	95	68	34	34
		7	56	32	68	41	42
			59	51	75	42	42
		10	66#	33	124	63	56*
			58	39	87	50	56*
		13	55	17	109	66	75*
			37	12	94	84	75*
		16	44	6	125	95	84*
			30	10	65	73	84*
Positive Control	3-Methylcholanthrene	2.5	28	16	334	398	395*
Positive Control			24	14	287	393	395*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	110	139	57	53
			82	111	108	44
			65	96	121	62
			66	83	98	50
Test Chemical	N-Phenyl-2-naphthylamine	1	76	92	102	45	51
			84	74	144	57	51
		4	80	72	168	70	67
			65	85	126	64	67
		7	57	46	162	96	83
			67	41	140	70	83
		10	66	24	297	151	136*
			59	25	213	120	136*
		13	57	9	327	193	193*
			59	14	345	194	193*
		16

Positive Control	3-Methylcholanthrene	2.5	69	84	480	234	233*
Positive Control			73	66	507	233	233*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination