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Study Information
Study Attribute Value
CEBS Accession Number: 002-02567-0001-0000-3
Chemical Name: N-Phenyl-p-phenylenediamine
CASRN: 101-54-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 887002_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Weakly Positive 
No Activation  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 09/18/1984    Trial Call: Weakly Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1049 511 0.487 10.22 26.5 0.000
Test Chemical N-Phenyl-p-phenylenediamine
 0.16      
50 1049 489 0.466 9.78 26.5 -4.305
 0.5       
50 1050 515 0.490 10.30 26.5 0.687
1.6       
50 1050 713 0.679 14.26 26.5 39.397
5         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C
 0.001     
50 1050 698 0.665 13.96 26.5 36.465
Mitomycin-C
 0.005     
10 208 309 1.486 30.90 26.5 204.965
Trend: 5.985        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 11/06/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1051 469 0.446 9.38 27.0 0.000
Test Chemical N-Phenyl-p-phenylenediamine
 0.5       
50 1047 461 0.440 9.22 27.0 -1.330
1         
50 1046 489 0.467 9.78 27.0 4.763
2         
50 1051 617 0.587 12.34 31.5 31.557
3         
50 1049 756 0.721 15.12 31.5 61.501
4         
0 0 0 0.000 0.00 31.5 N/A
Positive Control Mitomycin-C
 0.001     
50 1049 666 0.635 13.32 27.0 42.275
Mitomycin-C
 0.005     
10 210 339 1.614 33.90 27.0 261.751
Trend: 9.615        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/18/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1050 394 0.375 7.88 26.0 0.000
Test Chemical N-Phenyl-p-phenylenediamine
5         
50 1050 465 0.443 9.30 26.0 18.020
16         
50 1048 417 0.398 8.34 26.0 6.040
50         
50 1049 503 0.480 10.06 26.0 27.787
160         
50 1046 530 0.507 10.60 26.0 35.032
500         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
 0.3       
50 1051 689 0.656 13.78 26.0 74.707
Cyclophosphamide
 0.6       
10 210 179 0.852 17.90 26.0 127.157
Trend: 4.615        
Probability: 0.000