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Study Information
Study Attribute Value
CEBS Accession Number: 002-02567-0002-0000-4
Chemical Name: N-Phenyl-p-phenylenediamine
CASRN: 101-54-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 887002_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Questionable 
Induced Rat Liver S9  1_S9  Positive 
No Activation  Positive 

Trial #:1   Activation: No Activation    Date: 11/07/1984    Harvest Time: 13 hour(s)   Trial Call: Questionable   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 3 0.030 2.0 0 0.000 0.0 3 0.030 2.0 0 0.000 0.0
Test Chemical: N-Phenyl-p-phenylenediamine
 0.5       
100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
1         
100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
2.5       
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
5         
100 10 0.100 8.0 2 0.020 1.0 8 0.080 7.0 0 0.000 0.0
10         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C
 0.15      
100 31 0.310 16.0 6 0.060 4.0 15 0.150 12.0 10 0.100 1.0
Trend: 1.986 0.493 1.925  
Probability: 0.024 0.311 0.027  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 11/07/1984    Harvest Time: 16 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: N-Phenyl-p-phenylenediamine
10         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
25         
100 36 0.360 15.0 24 0.240 14.0 12 0.120 5.0 0 0.000 0.0
50         
100 27 0.270 15.0 18 0.180 12.0 9 0.090 7.0 0 0.000 0.0
75         
100 29 0.290 18.0 19 0.190 11.0 10 0.100 10.0 0 0.000 0.0
Positive Control: Cyclophosphamide
15         
100 45 0.450 33.0 26 0.260 22.0 18 0.180 15.0 1 0.010 1.0
Trend: 5.378 4.410 3.762  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 05/08/1985    Harvest Time: 17.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: N-Phenyl-p-phenylenediamine
2.5       
100 15 0.150 9.0 7 0.070 5.0 8 0.080 6.0 0 0.000 0.0
5         
100 45 0.450 10.0 19 0.190 8.0 5 0.050 4.0 21 0.210 3.0
7.5       
100 34 0.340 17.0 16 0.160 12.0 8 0.080 7.0 10 0.100 1.0
10         
100 111 1.110 36.0 58 0.580 28.0 13 0.130 9.0 40 0.400 4.0
Positive Control: Mitomycin-C
 0.0625    
100 22 0.220 17.0 12 0.120 11.0 10 0.100 8.0 0 0.000 0.0
Mitomycin-C
 0.25      
50 52 1.040 54.0 33 0.660 38.0 19 0.380 26.0 0 0.000 0.0
Trend: 6.768 6.097 2.592  
Probability: 0.000 0.000 0.005