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Study Information
Study Attribute Value
CEBS Accession Number: 002-02567-0006-0000-8
Chemical Name: N-Phenyl-p-phenylenediamine
CASRN: 101-54-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 867606
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 101 70 26 36
    83 98 98 40
    94 101 121 43
    90 100 98 36
Test Chemical N-Phenyl-p-phenylenediamine 
 0.5       
 54 61 76 47 49
    64 65 97 51
  
1         
 67 53 171 85 74*
    55 45 105 63
  
2         
 46 17 343 247 245*
    44 15 320 242
  
4         
   
Positive Control Methyl Methane Sulfonate 
15         
 48 32 200 139 130*
    52 28 189 120

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75 111 108 48 44
    76 118 88 38
    62 84 69 37
    66 87 104 52
Test Chemical N-Phenyl-p-phenylenediamine 
 0.25      
 69 86 159 77 61
    80 108 109 45
  
 0.5       
 81 105 127 52 56
    82 94 147 60
  
1         
 71 68 159 75 80*
    68 78 173 85
  
2         
 40 10 314 264 270*
    43 10 353 276
  
3         
   
Positive Control Methyl Methane Sulfonate 
15         
 24 17 162 228 285*
    31 17 317 343

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination