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Study Information
Study Attribute Value
CEBS Accession Number: 002-02574-0002-0000-2
Chemical Name: m-Nitrotoluene
CASRN: 99-08-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 713359_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 02/11/1982    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 4 0.040 3.0 1 0.010 1.0 3 0.030 2.0 0 0.000 0.0
Test Chemical: m-Nitrotoluene 
248         
 100 6 0.060 6.0 2 0.020 2.0 2 0.020 2.0 2 0.020 2.0 
299         
 100 5 0.050 5.0 0 0.000 0.0 5 0.050 5.0 0 0.000 0.0 
345         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 50 25 0.500 34.0 16 0.320 26.0 8 0.160 16.0 1 0.020 1.0
Trend: -0.087 -1.328 0.844  
Probability: 0.535 0.908 0.199  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/11/1982    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: m-Nitrotoluene 
437         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
460         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
483         
 100 6 0.060 6.0 1 0.010 1.0 5 0.050 4.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 50 20 0.400 18.0 6 0.120 12.0 3 0.060 6.0 11 0.220 2.0
Trend: 1.205 0.372 0.836  
Probability: 0.114 0.355 0.202  


Trial #:2   Activation: No Activation    Date: 01/21/1982    Harvest Time: 10.7 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 5 0.050 5.0 3 0.030 3.0 1 0.010 1.0 1 0.010 1.0
Test Chemical: m-Nitrotoluene 
150         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
300         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
398         
 50 2 0.040 4.0 1 0.020 2.0 1 0.020 2.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 50 21 0.420 22.0 8 0.160 8.0 3 0.060 4.0 10 0.200 2.0
Trend: -0.543 -0.643 0.619  
Probability: 0.706 0.740 0.268  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 01/21/1982    Harvest Time: 10.4 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 1 0.010 1.0 1 0.010 1.0 1 0.010 1.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: m-Nitrotoluene 
150         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
300         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
398         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 50 1.000 42.0 10 0.200 20.0 20 0.400 30.0 20 0.400 2.0
Trend: 0.434 0.151 0.431  
Probability: 0.332 0.440 0.333