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Study Information
Study Attribute Value
CEBS Accession Number: 002-02575-0001-0000-2
Chemical Name: o-Nitrotoluene
CASRN: 88-72-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 573604_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Questionable 
Induced Rat Liver S9  1_S9   
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 10/11/1981    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1035 485 0.469 9.70 25.5 0.000
Test Chemical o-Nitrotoluene 
117         
 50 1039 569 0.548 11.38 34.0 16.868 
176         
 50 1021 557 0.546 11.14 34.0 16.420 
218         
 50 1035 576 0.557 11.52 34.0 18.763 
282         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1037 2236 2.156 44.72 25.5 360.142
Trend: 2.561        
Probability: 0.005        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/11/1981    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 397 0.380 7.94 25.5 0.000
Test Chemical o-Nitrotoluene 
391         
 50 1049 442 0.421 8.84 25.5 10.910
Positive Control Cyclophosphamide 
1.5       
 50 1046 1537 1.469 30.74 25.5 286.784
Trend: 0.000        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 10/29/1981    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 380 0.362 7.60 25.5 0.000
Test Chemical o-Nitrotoluene 
354.83      
 50 1050 499 0.475 9.98 25.5 31.316 
380.95      
 50 1050 467 0.445 9.34 25.5 22.895 
423.28      
 50 1047 488 0.466 9.76 25.5 28.789
Positive Control Cyclophosphamide 
1.5       
 50 1049 1651 1.574 33.02 25.5 334.888
Trend: 3.110        
Probability: 0.001