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Study Information
Study Attribute Value
CEBS Accession Number: 002-02576-0001-0000-3
Chemical Name: p-Nitrotoluene
CASRN: 99-99-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 961037_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Weakly Positive 
No Activation  Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Weakly Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 02/28/1982    Trial Call: Weakly Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1038 487 0.469 9.74 25.5 0.000
Test Chemical p-Nitrotoluene
50         
43 889 382 0.430 8.88 25.5 -8.414
167         
50 1024 576 0.563 11.52 32.8 19.892
500         
50 1018 637 0.626 12.74 32.8 33.371
Positive Control Mitomycin-C
 0.005     
25 520 798 1.535 31.92 25.5 227.091
Trend: 5.850        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 03/23/1982    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1036 532 0.514 10.64 25.5 0.000
Test Chemical p-Nitrotoluene
200         
50 1035 732 0.707 14.64 35.8 37.727
300         
50 1032 698 0.676 13.96 35.8 31.712
400         
50 1034 803 0.777 16.06 35.8 51.232
500         
0 0 0 0.000 0.00 35.8 N/A
Positive Control Mitomycin-C
 0.005     
25 522 596 1.142 23.84 25.5 122.343
Trend: 6.482        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/28/1982    Trial Call: Negative   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1042 467 0.448 9.34 25.5 0.000
Test Chemical p-Nitrotoluene
50         
50 1043 464 0.445 9.28 25.5 -0.738
167         
50 1044 480 0.460 9.60 25.5 2.587
500         
50 1031 510 0.495 10.20 25.5 10.373
Positive Control Cyclophosphamide
1.5       
25 525 1082 2.061 43.28 25.5 359.853
Trend: 1.622        
Probability: 0.052        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/09/1982    Trial Call: Weakly Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1041 467 0.449 9.34 25.5 0.000
Test Chemical p-Nitrotoluene
600         
50 1027 535 0.521 10.70 25.5 16.123
700         
50 1030 657 0.638 13.14 35.5 42.188
Positive Control Cyclophosphamide
1.5       
25 517 654 1.265 26.16 25.5 181.982
Trend: 5.882        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 03/23/1982    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1047 451 0.431 9.02 25.5 0.000
Test Chemical p-Nitrotoluene
550         
50 1038 905 0.872 18.10 25.5 102.405
600         
50 1032 787 0.763 15.74 25.5 77.037
650         
50 1039 886 0.853 17.72 25.5 97.965
Positive Control Cyclophosphamide
1.5       
25 521 872 1.674 34.88 25.5 288.552
Trend: 9.790        
Probability: 0.000