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Study Information
Study Attribute Value
CEBS Accession Number: 002-02576-0001-0000-3
Chemical Name: p-Nitrotoluene
CASRN: 99-99-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 961037_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Weakly Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 02/28/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1038 487 0.469 9.74 25.5 0.000
Test Chemical p-Nitrotoluene 
50         
 43 889 382 0.430 8.88 25.5 -8.414 
167         
 50 1024 576 0.563 11.52 32.8 19.892 
500         
 50 1018 637 0.626 12.74 32.8 33.371
Positive Control Mitomycin-C 
 0.005     
 25 520 798 1.535 31.92 25.5 227.091
Trend: 5.850        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 03/23/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 532 0.514 10.64 25.5 0.000
Test Chemical p-Nitrotoluene 
200         
 50 1035 732 0.707 14.64 35.8 37.727 
300         
 50 1032 698 0.676 13.96 35.8 31.712 
400         
 50 1034 803 0.777 16.06 35.8 51.232 
500         
 0 0 0 0.000 0.00 35.8 N/A
Positive Control Mitomycin-C 
 0.005     
 25 522 596 1.142 23.84 25.5 122.343
Trend: 6.482        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/28/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 467 0.448 9.34 25.5 0.000
Test Chemical p-Nitrotoluene 
50         
 50 1043 464 0.445 9.28 25.5 -0.738 
167         
 50 1044 480 0.460 9.60 25.5 2.587 
500         
 50 1031 510 0.495 10.20 25.5 10.373
Positive Control Cyclophosphamide 
1.5       
 25 525 1082 2.061 43.28 25.5 359.853
Trend: 1.622        
Probability: 0.052        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/09/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1041 467 0.449 9.34 25.5 0.000
Test Chemical p-Nitrotoluene 
600         
 50 1027 535 0.521 10.70 25.5 16.123 
700         
 50 1030 657 0.638 13.14 35.5 42.188
Positive Control Cyclophosphamide 
1.5       
 25 517 654 1.265 26.16 25.5 181.982
Trend: 5.882        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 03/23/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 451 0.431 9.02 25.5 0.000
Test Chemical p-Nitrotoluene 
550         
 50 1038 905 0.872 18.10 25.5 102.405 
600         
 50 1032 787 0.763 15.74 25.5 77.037 
650         
 50 1039 886 0.853 17.72 25.5 97.965
Positive Control Cyclophosphamide 
1.5       
 25 521 872 1.674 34.88 25.5 288.552
Trend: 9.790        
Probability: 0.000