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Study Information
Study Attribute Value
CEBS Accession Number: 002-02576-0002-0000-4
Chemical Name: p-Nitrotoluene
CASRN: 99-99-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 961037_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Weakly Positive* 

Trial #:1   Activation: No Activation    Date: 04/06/1982    Harvest Time: 21 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 7 0.070 6.0 3 0.030 3.0 1 0.010 1.0 3 0.030 3.0
Test Chemical: p-Nitrotoluene 
300         
 100 9 0.090 8.0 3 0.030 3.0 5 0.050 5.0 1 0.010 1.0 
400         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 6 0.060 5.0 0 0.000 0.0 6 0.060 5.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.062     
 50 30 0.600 44.0 22 0.440 34.0 8 0.160 14.0 0 0.000 0.0
Trend: -1.265 -2.246 0.850  
Probability: 0.897 0.988 0.198  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/06/1982    Harvest Time: 21 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 13 0.130 4.0 0 0.000 0.0 3 0.030 3.0 10 0.100 1.0
Dimethyl Sulfoxide 
0         
 100 7 0.070 7.0 3 0.030 3.0 2 0.020 2.0 2 0.020 2.0
Test Chemical: p-Nitrotoluene 
400         
 100 3 0.030 3.0 1 0.010 1.0 1 0.010 1.0 1 0.010 1.0 
500         
 100 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 
550         
 100 23 0.230 21.0 5 0.050 5.0 18 0.180 17.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 50 14 0.280 26.0 7 0.140 12.0 7 0.140 14.0 0 0.000 0.0
Trend: 2.783 0.654 3.904  
Probability: 0.003 0.257 0.000  


Trial #:2   Activation: No Activation    Date: 03/18/1982    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 11 0.110 10.0 6 0.060 6.0 4 0.040 3.0 1 0.010 1.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 1 0.010 1.0
Test Chemical: p-Nitrotoluene 
300         
 100 7 0.070 7.0 5 0.050 5.0 2 0.020 2.0 0 0.000 0.0 
400         
 100 10 0.100 10.0 3 0.030 3.0 5 0.050 5.0 2 0.020 2.0 
500         
 100 9 0.090 9.0 3 0.030 3.0 6 0.060 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.062     
 50 24 0.480 32.0 11 0.220 16.0 10 0.200 16.0 3 0.060 6.0
Trend: 1.845 0.117 2.667  
Probability: 0.032 0.454 0.004  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 03/18/1982    Harvest Time: 20.3 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 24 0.240 9.0 4 0.040 4.0 9 0.090 4.0 11 0.110 2.0
Dimethyl Sulfoxide 
0         
 100 9 0.090 8.0 5 0.050 5.0 3 0.030 3.0 1 0.010 1.0
Test Chemical: p-Nitrotoluene 
500         
 100 12 0.120 7.0 4 0.040 4.0 8 0.080 4.0 0 0.000 0.0 
550         
 100 10 0.100 8.0 5 0.050 5.0 5 0.050 4.0 0 0.000 0.0 
600         
 100 30 0.300 24.0 5 0.050 5.0 25 0.250 22.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 50 40 0.800 44.0 20 0.400 34.0 9 0.180 16.0 11 0.220 2.0
Trend: 3.363 0.107 4.580  
Probability: 0.000 0.457 0.000