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Study Information
Study Attribute Value
CEBS Accession Number: 002-02576-0023-0000-7
Chemical Name: p-Nitrotoluene
CASRN: 99-99-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 762326
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 151r 153 101 22 31
    100 108 89 30
    90 104 92 34
    68 89 60 29
Test Chemical p-Nitrotoluene 
75         
 84# 79 50 20 25
    90 66 73 27
    56 61 45 27
  
100         
 61 44 50 27 26
    58# 51 51 30
    81 72 53 22
  
150         
 81 81 69 28 31
    67 52 68 34
  
180         
 72 38 82 38 38
    86 41 124 48
    85# 48 70 27
  
200         
 58 32 53 30 42
    52 22 79 50
    59 29 81 46
  
240         
 46 21 77 56 49*
    51 19 68 45
    30 14 42 47
Positive Control Methyl Methane Sulfonate 
5         
 47# 30 492 349 398*
    47# 28 511 361
    37 24 534 483

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 121r 110 66 18 18
    103 121 54 17
    83# 102 48 19
    81 77 44 18
Test Chemical p-Nitrotoluene 
25         
 109 87 58 18 20
    113# 82 71 21
    109 81 73 22
  
50         
 128r 77 48 12
    122r 88 46 13
    116# 72 77 22
  
75         
 109 73 45 14 15
    104 56 49 16
    124r 76 74 20
  
100         
 59 38 70 40 25
    103 56 60 19
    114# 50 58 17
  
150         
 94 38 67 24 29*
    76 39 79 35
    95 43 79 28
  
250         
 80 24 53 22 35*
    60 13 66 37
    36 11 50 47
Positive Control Methyl Methane Sulfonate 
5         
 68 42 405 198 159*
    96 55 345 120

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 105 101 77 24 22
    105 114 59 19
    97 105 52 18
    88 81 70 26
Test Chemical p-Nitrotoluene 
50         
 84 77 105 42 35*
    83 93 63 25
    87 76 96 37
  
75         
 94 74 78 28 35*
    68 63 77 38
    82 68 100 41
  
100         
 102 71 130 42 41*
    86 61 87 34
    87 57 120 46
  
150         
 68 57 85 42 42*
    80 63 85 35
    77 60 111 48
  
200         
 80# 40 129 54 49*
    97 53 109 38
    103 42 170 55
  
300         
 108 41 141 44 35
    169r 78 126 25
    91 43 71 26
Positive Control 3-Methylcholanthrene 
2.5       
 75 42 319 143 134*
    91# 48 328 120
    81 50 337 140

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 94 94 72 26 25
    104 115 71 23
    90 84 66 24
    91 107 69 25
Test Chemical p-Nitrotoluene 
50         
 86 83 64 25 27
    72 102 61 28
    101 81 86 28
  
75         
 104 80 68 22 23
    105 88 65 21
    72 77 60 28
  
100         
 109 89 113 35 29
    117 79 91 26
    108 86 82 25
  
150         
 104 78 87 28 32
    108 98 112 34
    111 69 113 34
  
200         
 112 67 123 36 35
    109 71 120 37
    90 72 89 33
  
300         
 110 45 106 32 40*
    106 57 122 38
    99 47 149 50
Positive Control 3-Methylcholanthrene 
2.5       
 85 68 501 198 203*
    98 109 363 124
    56 51 484 286

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 101 87 122 40 41
    104 90 128 41
    102 108 139 45
    79 115 85 36
Test Chemical p-Nitrotoluene 
50         
 120 65 178 49 58
    110 68 185 56
    107 70 219 69
  
75         
 112 48 175 52 70*
    103 69 263 85
    84 61 181 72
  
100         
 111 44 234 70 70*
    113 62 263 78
    86 37 162 63
  
150         
 87 31 205 79 96*
    105 39 380 120
    93 35 251 90
  
200         
 90 22 353 131 150*
    86 17 436 170
  
300         
 38 4 479 424 266*
    84 13 472 187
    75 8 418 186
  
500         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 67# 34 995 497 556*
    55 21 1031 623
    51# 21 842 549

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 118 108 118 33 34
    106 101 93 29
    88 91 108 41
Test Chemical p-Nitrotoluene 
50         
 108 61 139 43 52*
    109 82 144 44
    77 52 160 70
  
100         
 97 52 180 62 62*
    121r 65 157 43
    108 59 204 63
  
150         
 97 45 189 65 51
    122r 48 111 30
    111 50 124 37
  
200         
 88 31 316 119 85*
    118 39 233 66
    98 40 205 70
  
250         
 103 28 194 63 83*
    105 32 249 79
    94 29 300 107
  
300         
 83 13 388 156 108*
    94 29 192 68
    92 19 272 98
Positive Control 3-Methylcholanthrene 
2.5       
 91 43 726 266 294*
    92 41 952 346
    96 65 785 272

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination